Coupling site-directed mutagenesis with high-level expression: large scale production of mutant porins from E. coli.

Coupling site-directed mutagenesis with high-level expression: large scale production of mutant porins from E. coli. Research Abstract Details 

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Coupling site-directed mutagenesis with high-level expression: large scale production of mutant porins from E. coli. Abstract Text:

A Prilipov,P S Phale,P Van Gelder,J P Rosenbusch,R Koebnik,

Combination of an origin repair mutagenesis system with a new mutS host strain increased the efficiency of mutagenesis from 46% to 75% mutant clones. Overexpression with the T7 expression system afforded large quantities of proteins from mutant strains. A series of E. coli BE host strains devoid of major outer membrane proteins was constructed, facilitating the purification of mutant porins to homogeneity. This allowed preparation of 149 porin mutants in E. coli used in detailed explorations of the structure and function of this membrane protein to high resolution.

Coupling site-directed mutagenesis with high-level expression: large scale production of mutant porins from E. coli. Publishing Authors By Initials

A Prilipov,PS Phale,P Van Gelder,JP Rosenbusch,R Koebnik,

For similar proteins: viral proteins research abstracts see: proteins: viral proteins research

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Coupling site-directed mutagenesis with high-level expression: large scale production of mutant porins from E. coli. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: FEMS microbiology letters

VOLUME: 163

Page Numbers: 65-72

Journal Abbreviation: FEMS Microbiol. Lett.

ISSN: 0378-1097

DAY: 1

MONTH: Jun

YEAR: 1998

Coupling site-directed mutagenesis with high-level expression: large scale production of mutant porins from E. coli. Information

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LANGUAGE: eng

NlmUniqueID: 7705721

Coupling site-directed mutagenesis with high-level expression: large scale production of mutant porins from E. coli. Keywords Mesh Terms:

KEYWORDS: Viral Proteins

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Coupling site-directed mutagenesis with high-level expression: large scale production of mutant porins from E. coli. Information

Substance Name: MutS protein, E coli

Registry Number: EC 3.6.1.3

Grant and Affiliation Information for Coupling site-directed mutagenesis with high-level expression: large scale production of mutant porins from E. coli.

AFFILIATION: Department of Microbiology, Biozentrum, University of Basel, Switzerland.

Country: NETHERLANDS

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MEDLINETA: FEMS Microbiol Lett

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