Coupling of fatty acid and phospholipid synthesis in Bacillus subtilis.

Coupling of fatty acid and phospholipid synthesis in Bacillus subtilis. Research Abstract Details 

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Coupling of fatty acid and phospholipid synthesis in Bacillus subtilis. Abstract Text:

Luciana Paoletti,Ying-Jie Lu,Gustavo E Schujman,Diego de Mendoza,Charles O Rock,

plsX (acyl-acyl carrier protein [ACP]:phosphate acyltransferase), plsY (yneS) (acyl-phosphate:glycerol-phosphate acyltransferase), and plsC (yhdO) (acyl-ACP:1-acylglycerol-phosphate acyltransferase) function in phosphatidic acid formation, the precursor to membrane phospholipids. The physiological functions of these genes was inferred from their in vitro biochemical activities, and this study investigated their roles in gram-positive phospholipid metabolism through the analysis of conditional knockout strains in the Bacillus subtilis model system. The depletion of PlsX led to the cessation of both fatty acid synthesis and phospholipid synthesis. The inactivation of PlsY also blocked phospholipid synthesis, but fatty acid formation continued due to the appearance of acylphosphate intermediates and fatty acids arising from their hydrolysis. Phospholipid synthesis ceased following PlsC depletion, but fatty acid synthesis continued at a high rate, leading to the accumulation of fatty acids arising from the dephosphorylation of 1-acylglycerol-3-P followed by the deacylation of monoacylglycerol. Analysis of glycerol 3-P acylation in B. subtilis membranes showed that PlsY was an acylphosphate-specific acyltransferase, whereas PlsC used only acyl-ACP as an acyl donor. PlsX was found in the soluble fraction of disrupted cells but was associated with the cell membrane in intact organisms. These data establish that PlsX is a key enzyme that coordinates the production of fatty acids and membrane phospholipids in B. subtilis.

Coupling of fatty acid and phospholipid synthesis in Bacillus subtilis. Publishing Authors By Initials

L Paoletti,YJ Lu,GE Schujman,D de Mendoza,CO Rock,

For similar lipids: membrane lipids: phospholipids research abstracts see: lipids: membrane lipids: phospholipids research

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Coupling of fatty acid and phospholipid synthesis in Bacillus subtilis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of bacteriology

VOLUME: 189

Page Numbers: 5816-24

Journal Abbreviation: J. Bacteriol.

ISSN: 0021-9193

DAY: 8

MONTH: 06

YEAR: 2007

Coupling of fatty acid and phospholipid synthesis in Bacillus subtilis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985120

Coupling of fatty acid and phospholipid synthesis in Bacillus subtilis. Keywords Mesh Terms:

KEYWORDS: Phospholipids

MESH TERMS: biosynthesis

Chemical & Substance for Abstract: Coupling of fatty acid and phospholipid synthesis in Bacillus subtilis. Information

Substance Name: plsX protein, bacteria

Registry Number: 0

Grant and Affiliation Information for Coupling of fatty acid and phospholipid synthesis in Bacillus subtilis.

AFFILIATION: Department of Infectious Diseases, St. Jude Children's Research Hospital, 332 N. Lauderdale, Memphis, TN 38105-2794, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM 34496

ACRONYM: GM

MEDLINETA: J Bacteriol

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