Coupled rolling circle amplification loop-mediated amplification for rapid detection of short DNA sequences.

Coupled rolling circle amplification loop-mediated amplification for rapid detection of short DNA sequences. Research Abstract Details 

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Coupled rolling circle amplification loop-mediated amplification for rapid detection of short DNA sequences. Abstract Text:

Circularizable oligonucleotide probes can detect short DNA sequences with single-base resolution at the site of ligation and can be amplified by rolling circle amplification (RCA) using strand displacing polymerases. A secondary amplification scheme was developed that uses the loop-mediated amplification reaction concurrent with RCA to achieve rapid signal development from the starting circular molecules. This isothermal reaction was found to be significantly faster than the comparable hyperbranching amplification method and could detect 100 circular copies in less than 1 h.

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Coupled rolling circle amplification loop-mediated amplification for rapid detection of short DNA sequences. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: BioTechniques

VOLUME: 45

Page Numbers: 275-80

Journal Abbreviation: BioTechniques

ISSN: 0736-6205

DAY: 9

MONTH: Sep

YEAR: 2008

Coupled rolling circle amplification loop-mediated amplification for rapid detection of short DNA sequences. Information

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LANGUAGE: eng

NlmUniqueID: 8306785

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Grant and Affiliation Information for Coupled rolling circle amplification loop-mediated amplification for rapid detection of short DNA sequences.

AFFILIATION: Department of Bioengineering, University of California San Diego, La Jolla, CA, USA.

Country: England

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MEDLINETA: Biotechniques

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