Correlation of the expression level of C1q mRNA and the number of C1q-positive plaques in the Alzheimer Disease temporal cortex. analysis of C1q mrna and its protein using adjacent or nearby sections.

Correlation of the expression level of C1q mRNA and the number of C1q-positive plaques in the Alzheimer Disease temporal cortex. analysis of C1q mrna and its protein using adjacent or nearby sections. Research Abstract Details 

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Correlation of the expression level of C1q mRNA and the number of C1q-positive plaques in the Alzheimer Disease temporal cortex. analysis of C1q mrna and its protein using adjacent or nearby sections. Abstract Text:

I Tooyama,H Sato,O Yasuhara,H Kimura,Y Konishi,Y Shen,D G Walker,T G Beach,L I Sue,J Rogers,

We compared the expression level of C1q mRNA and the number of C1q-positive plaques in adjacent or nearby brain sections from Alzheimer disease (AD) and control cases. Small blocks of temporal cortex were fixed with 4% paraformaldehyde for 2 days at 4 degrees C. After cryoprotection with solutions containing 10-20% glycerol and 2% dimethylsulfoxide, 40-microm sections were cut from the tissue blocks. A section from each case was stained by immunohistochemistry using a C1q antibody, while RNA was purified from adjacent or nearby sections using a combination of proteinase K pretreatment followed by extraction using Trizol reagent. The expression of C1q B chain mRNA was analyzed in these samples by the reverse-transcription polymerase chain reaction (RT-PCR). The intensities of the PCR products were measured by an image analyzer. The expression of C1q B chain mRNA was significantly more abundant in AD than in control cases (p < 0.05). Immunohistochemical analysis showed that C1q protein was localized in senile plaques in the AD brain. The number of C1q-positive plaques correlated with the expression level of C1q gene (p < 0.05). The present results suggest that C1q protein in senile plaques originates is endogenously produced in the AD brain.

Correlation of the expression level of C1q mRNA and the number of C1q-positive plaques in the Alzheimer Disease temporal cortex. analysis of C1q mrna and its protein using adjacent or nearby sections. Publishing Authors By Initials

I Tooyama,H Sato,O Yasuhara,H Kimura,Y Konishi,Y Shen,DG Walker,TG Beach,LI Sue,J Rogers,

For similar nervous system: central nervous system: brain: prosencephalon: telencephalon: cerebrum: cerebral cortex: temporal lobe research abstracts see: nervous system: central nervous system: brain: prosencephalon: telencephalon: cerebrum: cerebral cortex: temporal lobe research

PUBMED ID PMID:

MEDLINE DATE: 2001 Jul-Aug

Correlation of the expression level of C1q mRNA and the number of C1q-positive plaques in the Alzheimer Disease temporal cortex. analysis of C1q mrna and its protein using adjacent or nearby sections. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Dementia and geriatric cognitive disorders

VOLUME: 12

Page Numbers: 237-42

Journal Abbreviation: Dement Geriatr Cogn Disord

ISSN: 1420-8008

DAY: 24

MONTH: 03

YEAR: 2008

Correlation of the expression level of C1q mRNA and the number of C1q-positive plaques in the Alzheimer Disease temporal cortex. analysis of C1q mrna and its protein using adjacent or nearby sections. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9705200

Correlation of the expression level of C1q mRNA and the number of C1q-positive plaques in the Alzheimer Disease temporal cortex. analysis of C1q mrna and its protein using adjacent or nearby sections. Keywords Mesh Terms:

KEYWORDS: Temporal Lobe

MESH TERMS: pathology

Chemical & Substance for Abstract: Correlation of the expression level of C1q mRNA and the number of C1q-positive plaques in the Alzheimer Disease temporal cortex. analysis of C1q mrna and its protein using adjacent or nearby sections. Information

Substance Name: Complement C1q

Registry Number: 80295-33-6

Grant and Affiliation Information for Correlation of the expression level of C1q mRNA and the number of C1q-positive plaques in the Alzheimer Disease temporal cortex. analysis of C1q mrna and its protein using adjacent or nearby sections.

AFFILIATION: Molecular Neuroscience Research Center, Shiga University of Medical Science, Otsu, Japan. kinchan@sums.shiga-med.ac.jp

Country: Switzerland

AGENCY: United States NIA

GRANT: AGO7367

ACRONYM: AG

MEDLINETA: Dement Geriatr Cogn Disord

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