Correlation of in vitro cytotoxicity with paracellular permeability in mortal rat intestinal cells.

Correlation of in vitro cytotoxicity with paracellular permeability in mortal rat intestinal cells. Research Abstract Details 

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Correlation of in vitro cytotoxicity with paracellular permeability in mortal rat intestinal cells. Abstract Text:

Roula Konsoula,Frank A Barile,

INTRODUCTION: The rat small intestinal cell line, IEC-18, was used as an in vitro model to differentiate between acute cytotoxicity (AC) and paracellular permeability (PP) of selected chemicals. METHODS: This study compares the low resistance rat intestinal mortal cell line, IEC-18 (transepithelial electrical resistance, TEER=160+/-10 Omega cm(2)) with the high resistance human intestinal cell line, Caco-2 (TEER=900+/-100 Omega cm(2)). The two cell lines differ in state of differentiation, TEER and paracellular permeability characteristics. The IEC-18 cell line is originated from the ileum and resembles more closely the small intestine than the Caco-2. Cytotoxicity was carried out using MTT cell viability assay in 96-well plates for 24-h exposure time. PP was measured using TEER (membrane integrity indicator) and PP markers such as [(3)H]-D-mannitol, lucifer yellow (LY) and FITC-dextran (fluorescein-dextran) on cells grown on inserts. RESULTS: The data showed that there is a high correlation (R(2)=0.99) between MTT and TEER using IEC-18 cell for 24-h exposure time. IEC-18 is as sensitive as Caco-2 for both MTT and TEER measurements. Decrease in TEER is inversely proportional with increase in PP of tight junction indicators. There is a good correlation between IC(50)'s MTT, TEER and Registry of Cytotoxicity (RC) data. DISCUSSION: Based on the results from the experiments, IEC-18 can be used as an in vitro model to differentiate between concentrations needed for AC and those required for PP.

Correlation of in vitro cytotoxicity with paracellular permeability in mortal rat intestinal cells. Publishing Authors By Initials

R Konsoula,FA Barile,

For similar pharmaceutical preparations: xenobiotics research abstracts see: pharmaceutical preparations: xenobiotics research

PUBMED ID PMID:

MEDLINE DATE:

Correlation of in vitro cytotoxicity with paracellular permeability in mortal rat intestinal cells. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of pharmacological and toxicological metho

VOLUME: 55

Page Numbers: 176-83

Journal Abbreviation:

ISSN: 1056-8719

DAY: 16

MONTH: 06

YEAR: 2006

Correlation of in vitro cytotoxicity with paracellular permeability in mortal rat intestinal cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9206091

Correlation of in vitro cytotoxicity with paracellular permeability in mortal rat intestinal cells. Keywords Mesh Terms:

KEYWORDS: Xenobiotics

MESH TERMS: toxicity

Chemical & Substance for Abstract: Correlation of in vitro cytotoxicity with paracellular permeability in mortal rat intestinal cells. Information

Substance Name: Dextrans

Registry Number: 9004-54-0

Grant and Affiliation Information for Correlation of in vitro cytotoxicity with paracellular permeability in mortal rat intestinal cells.

AFFILIATION: College of Pharmacy and Allied Health Professions, Department of Pharmaceutical Sciences, St. John's University, 8000 Utopia Parkway, Jamaica, NY 11439, USA.

Country: United States

AGENCY: United States NIEHS

GRANT: R15 ES 012170-01

ACRONYM: ES

MEDLINETA: J Pharmacol Toxicol Methods

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