Correlation of developmental differences of nuclear transfer embryos cells to the methylation profiles of nuclear transfer donor cells in Swine.

Correlation of developmental differences of nuclear transfer embryos cells to the methylation profiles of nuclear transfer donor cells in Swine. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Correlation of developmental differences of nuclear transfer embryos cells to the methylation profiles of nuclear transfer donor cells in Swine. Abstract Text:

Aaron J Bonk,Hee-Tae Cheong,Rongfeng Li,Liangxue Lai,Yanhong Hao,Zhonghua Liu,Melissa Samuel,Emily A Fergason,Kristin M Whitworth,Clifton N Murphy,Eric Antoniou,Randall S Prather,Aaron J Bonk,Hee-Tae Cheong,Rongfeng Li,Liangxue Lai,Yanhong Hao,Zhonghua Liu,Melissa Samuel,Emily A Fergason,Kristin M Whitworth,Clifton N Murphy,Eric Antoniou,Randall S Prather,

Methylation of DNA is the most commonly studied epigenetic mechanism of developmental competence and somatic cell nuclear transfer (SCNT). Previous studies of epigenetics and the SCNT procedures have examined the effects of different culture media on donor cells and reconstructed embryos, and the methylation status of specific genes in the fetus or live offspring. Here we used a microarray based approach to identify the methylation profiles of SCNT donor cells including three clonal porcine fetal fibroblast-like cell sublines and adult somatic cells selected from kidney and mammary tissues. The methylation profiles of the donor cells were then analyzed with respect to their ability to direct development to the blastocyst stage after nuclear transfer. Clonal cell lines A2, A7 and A8 had blastocyst rates of 11.7%(a), 16.7%(ab) and 20.0%(b), respectively ((ab) p < 0.05). Adult somatic cells included kidney, mammary (large), and mammary (small) also had different blastocyst rates (ab p < 0.05) of 4.2% (a), 10.7% (ab) and 18.3% (b), respectively. For clonal donor cells and for adult somatic cell groups the donor cells with the highest blastocyst rates also had methylation profiles with the lowest similarity to the methylation profiles of the in vivo-produced blastocysts. Conversely, the donor cells with the lowest blastocyst rates had methylation profiles with the highest similarity to the methylation profiles of the in vivo-produced blastocysts. Our findings show there is an inverse correlation to the similarity of the methylation profiles of the donor cells and the in vivo-produced embryos, and to the blastocyst rates following SCNT.

Correlation of developmental differences of nuclear transfer embryos cells to the methylation profiles of nuclear transfer donor cells in Swine. Publishing Authors By Initials

AJ Bonk,HT Cheong,R Li,L Lai,Y Hao,Z Liu,M Samuel,EA Fergason,KM Whitworth,CN Murphy,E Antoniou,RS Prather,AJ Bonk,HT Cheong,R Li,L Lai,Y Hao,Z Liu,M Samuel,EA Fergason,KM Whitworth,CN Murphy,E Antoniou,RS Prather,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Correlation of developmental differences of nuclear transfer embryos cells to the methylation profiles of nuclear transfer donor cells in Swine. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Epigenetics : official journal of the DNA Methylat

VOLUME: 2

Page Numbers: 179-86

Journal Abbreviation:

ISSN: 1559-2308

DAY: 6

MONTH: 08

YEAR: 2007

Correlation of developmental differences of nuclear transfer embryos cells to the methylation profiles of nuclear transfer donor cells in Swine. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101265293

Correlation of developmental differences of nuclear transfer embryos cells to the methylation profiles of nuclear transfer donor cells in Swine. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Correlation of developmental differences of nuclear transfer embryos cells to the methylation profiles of nuclear transfer donor cells in Swine. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Correlation of developmental differences of nuclear transfer embryos cells to the methylation profiles of nuclear transfer donor cells in Swine.

AFFILIATION: Division of Animal Science, University of Missouri-Columbia, Columbia, Missouri, USA.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Epigenetics

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Correlation of developmental differences of nuclear transfer embryos cells to the methylation profiles of nuclear transfer donor cells in Swine Related Publications

• A protein tyrosine phosphatase inhibitor, sodium orthovanadate, causes parthenogenetic activation of pig oocytes via an increase in protein tyrosine kinase activity.
• Aberrant DNA methylation in porcine in vitro-, parthenogenetic-, and somatic cell nuclear transfer-produced blastocysts.
• An anisotropy of human tactile sensitivity and its relation to the visual oblique effect.
• Carbohydrate tolerance and serum lipid responses to type of dietary carbohydrate and oral contraceptive use in young women.
• Cloned transgenic heart-healthy pork?
• Cloned transgenic swine via in vitro production and cryopreservation.
• Concentration and composition of free amino acids and osmolalities of porcine oviductal and uterine fluid and their effects on development of porcine IVF embryos.
• Correlation of developmental differences of nuclear transfer embryos cells to the methylation profiles of nuclear transfer donor cells in Swine.
• Development and calcium level changes in pre-implantation porcine nuclear transfer embryos activated with 6-DMAP after fusion.
• Diacylglycerol-enhanced electrical activation of porcine oocytes matured in vitro.
• Electromagnetic modeling of active silicon nanocrystal waveguides.
• End note:summary.
• Fiber-to-waveguide coupler based on the parabolic reflector.
• High renal ectopy; a case report.
• Hyaluronic acid fillers for the male patient.
• Investigations of the diurnal cycle and mixing state of oxalic acid in individual particles in Asian aerosol outflow.
• Juvéderm: a hyaluronic acid dermal filler.
• Multichannel wavelength division multiplexing with photonic crystals.
• Nuclear remodeling and nuclear reprogramming for making transgenic pigs by nuclear transfer.
• Osteopontin improves in vitro development of porcine embryos and decreases apoptosis.
• Photoresist characterization and linearization procedure for the gray-scale fabrication of diffractive optical elements.
• Plasma fructose, uric acid, and inorganic phosphorus responses of hyperinsulinemic men fed fructose.
• Pluristem Therapeutics, Inc.
• Pneumonephrosis; a complication of necrotizing pyelonephritis.
• Real-time, single-particle measurements of oligomers in aged ambient aerosol particles.
• Surgical treatment of serous cyst of the kidney.
• Targeted genetic modification: xenotransplantation and beyond.
• The CB2 cannabinoid agonist AM-1241 prolongs survival in a transgenic mouse model of amyotrophic lateral sclerosis when initiated at symptom onset.
• Thin film solar cell design based on photonic crystal and diffractive grating structures.
• Vector-based plane-wave spectrum method for the propagation of cylindrical electromagnetic fields.