Correction of aberrant imprinting of IGF2 in human tumors by nuclear transfer-induced epigenetic reprogramming.

Correction of aberrant imprinting of IGF2 in human tumors by nuclear transfer-induced epigenetic reprogramming. Research Abstract Details 

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Correction of aberrant imprinting of IGF2 in human tumors by nuclear transfer-induced epigenetic reprogramming. Abstract Text:

Hui Ling Chen,Tao Li,Xin Wen Qiu,Jie Wu,Jian Qun Ling,Zhi Hong Sun,Weibo Wang,Wei Chen,Aiju Hou,Thanh H Vu,Andrew R Hoffman,Ji-Fan Hu,

Loss of genomic imprinting of insulin-like growth factor II (IGF2) is a hallmark of many human neoplasms. We attempted to correct this aberrant epigenotype by transferring nuclei from human tumor cells that showed loss of IGF2 imprinting into enucleated mouse and human fibroblasts that had maintained normal IGF2 imprinting. After nuclear transfer, the abnormal biallelic expression of IGF2 in tumor nuclei transiently converted to normal monoallelic imprinted expression in the reconstructed diploid cells. In tetraploid hybrid cells, however, normal IGF2 imprinting was permanently restored in the tumor genome. Inhibition of the synthesis of putative trans imprinting factors with cycloheximide led to loss of IGF2 imprinting in normal cultured fibroblasts, suggesting that normal cells produce proteins that act in trans to induce or maintain genomic imprinting. These data demonstrate that an abnormal tumor epigenotype can be corrected by in vitro reprogramming, and suggest that loss of imprinting is associated with the loss of activity of non-CTCF trans imprinting factor(s) that are either inactivated or mutated in tumors.

Correction of aberrant imprinting of IGF2 in human tumors by nuclear transfer-induced epigenetic reprogramming. Publishing Authors By Initials

HL Chen,T Li,XW Qiu,J Wu,JQ Ling,ZH Sun,W Wang,W Chen,A Hou,TH Vu,AR Hoffman,JF Hu,

For similar proteins: transcription factors: repressor proteins research abstracts see: proteins: transcription factors: repressor proteins research

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Correction of aberrant imprinting of IGF2 in human tumors by nuclear transfer-induced epigenetic reprogramming. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: The EMBO journal

VOLUME: 25

Page Numbers: 5329-38

Journal Abbreviation: EMBO J.

ISSN: 0261-4189

DAY: 2

MONTH: 11

YEAR: 2006

Correction of aberrant imprinting of IGF2 in human tumors by nuclear transfer-induced epigenetic reprogramming. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8208664

Correction of aberrant imprinting of IGF2 in human tumors by nuclear transfer-induced epigenetic reprogramming. Keywords Mesh Terms:

KEYWORDS: Repressor Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Correction of aberrant imprinting of IGF2 in human tumors by nuclear transfer-induced epigenetic reprogramming. Information

Substance Name: Insulin-Like Growth Factor II

Registry Number: 67763-97-7

Grant and Affiliation Information for Correction of aberrant imprinting of IGF2 in human tumors by nuclear transfer-induced epigenetic reprogramming.

AFFILIATION: Medical Service, VA Palo Alto Health Care System, Palo Alto, CA, USA.

Country: England

AGENCY: United States NCI

GRANT: R43 CA86664-01

ACRONYM: CA

MEDLINETA: EMBO J

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