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Coordination of levels of elongation factors Tu, Ts, and G, and ribosomal protein SI in Escherichia coli.

Coordination of levels of elongation factors Tu, Ts, and G, and ribosomal protein SI in Escherichia coli. Research Abstract Details 

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  • Coordination of levels of elongation factors Tu, Ts, and G, and ribosomal protein SI in Escherichia coli. Abstract Text:

    a miyajimaA Miyajima,y kaziroY Kaziro,

    The amounts of the polypeptide chain elongation factors Tu, Ts, and G, and ribosomal protein SI were assessed under various growth conditions using three independent procedures: (a) Immunoprecipitation and gel electrophoresis, (b) radioimmune assay, and (c) activity measurements. It was demonstrated that, during balanced growth of E. coli, the intracellular levels of these proteins increased in proportion to the growth rate, and the ratio of EF-Tu:EF-Ts:EF-G:protein SI was 4-5:1:1:1, at all growth rates. The effects of isoleucine starvation on the rates of synthesis of these proteins were examined using a pair of isogenic stringent and relaxed strains. The syntheses of all these proteins were found to be under the influence of stringent control. These results indicate that in E. coli the syntheses of the above four proteins are regulated in a coordinated manner and are subject to stringent control.

    Coordination of levels of elongation factors Tu, Ts, and G, and ribosomal protein SI in Escherichia coli. Publishing Authors By Initials

    a miyajimaA Miyajima,y kaziroY Kaziro,

    For similar amino acids, peptides, and proteins: amino acids: amino acids, branched-chain: valine research abstracts see: amino acids, peptides, and proteins: amino acids: amino acids, branched-chain: valine research

    PUBMED ID PMID:

    MEDLINE DATE:

    Coordination of levels of elongation factors Tu, Ts, and G, and ribosomal protein SI in Escherichia coli. Journal Published:

    PUBLICATION TYPE: Journal Article

    Journal: Journal of biochemistry

    VOLUME: 83

    Page Numbers: 453-62

    Journal Abbreviation: J. Biochem.

    ISSN: 0021-924X

    DAY: 19

    MONTH: Feb

    YEAR: 1978

    Coordination of levels of elongation factors Tu, Ts, and G, and ribosomal protein SI in Escherichia coli. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 376600

    Coordination of levels of elongation factors Tu, Ts, and G, and ribosomal protein SI in Escherichia coli. Keywords Mesh Terms:

    KEYWORDS: Valine

    MESH TERMS: metabolism

    Chemical & Substance for Abstract: Coordination of levels of elongation factors Tu, Ts, and G, and ribosomal protein SI in Escherichia coli. Information

    Substance Name: Isoleucine

    Registry Number: 73-32-5

    Grant and Affiliation Information for Coordination of levels of elongation factors Tu, Ts, and G, and ribosomal protein SI in Escherichia coli.

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    Country: JAPAN

    JAPAN Research PublicationJAPAN Research Publication

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    MEDLINETA: J Biochem

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