The amounts of the polypeptide chain elongation factors Tu, Ts, and G, and ribosomal protein SI were assessed under various growth conditions using three independent procedures: (a) Immunoprecipitation and gel electrophoresis, (b) radioimmune assay, and (c) activity measurements. It was demonstrated that, during balanced growth of E. coli, the intracellular levels of these proteins increased in proportion to the growth rate, and the ratio of EF-Tu:EF-Ts:EF-G:protein SI was 4-5:1:1:1, at all growth rates. The effects of isoleucine starvation on the rates of synthesis of these proteins were examined using a pair of isogenic stringent and relaxed strains. The syntheses of all these proteins were found to be under the influence of stringent control. These results indicate that in E. coli the syntheses of the above four proteins are regulated in a coordinated manner and are subject to stringent control.
Coordination of levels of elongation factors Tu, Ts, and G, and ribosomal protein SI in Escherichia coli. Publishing Authors By Initials
Coordination of levels of elongation factors Tu, Ts, and G, and ribosomal protein SI in Escherichia coli. Journal Published:
PUBLICATION TYPE: Journal Article
Journal: Journal of biochemistry
VOLUME: 83
Page Numbers: 453-62
Journal Abbreviation: J. Biochem.
ISSN: 0021-924X
DAY: 19
MONTH: Feb
YEAR: 1978
Coordination of levels of elongation factors Tu, Ts, and G, and ribosomal protein SI in Escherichia coli. Information
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LANGUAGE: eng
NlmUniqueID: 376600
Coordination of levels of elongation factors Tu, Ts, and G, and ribosomal protein SI in Escherichia coli. Keywords Mesh Terms:
KEYWORDS: Valine
MESH TERMS: metabolism
Chemical & Substance for Abstract: Coordination of levels of elongation factors Tu, Ts, and G, and ribosomal protein SI in Escherichia coli. Information
Substance Name: Isoleucine
Registry Number: 73-32-5
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Country: JAPAN
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MEDLINETA: J Biochem
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