Cooperative transcription activation between Ad1, a CRE-like element, and other elements in the CYP11B gene promoter.

Cooperative transcription activation between Ad1, a CRE-like element, and other elements in the CYP11B gene promoter. Research Abstract Details 

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Cooperative transcription activation between Ad1, a CRE-like element, and other elements in the CYP11B gene promoter. Abstract Text:

T Hashimoto,K Morohashi,K Takayama,S Honda,T Wada,H Handa,T Omura,

We previously reported the presence of six different cis-acting elements (Ad1 to Ad6) in the promoter region of the bovine CYP11B gene. Although the Ad1 site (TGACGTGA) was similar to a palindromic CRE (TGACGTCA), two other upstream sequences, Ad3 and Ad4, were identified as the cAMP response sequences of the gene. We analyzed the functional relationship between the Ad1 site and the upstream elements. Mutation analyses of the Ad1 site indicated that the 5' half of the site (TGACG) was important for the transcription of the gene in vitro. In Y-1 cells, a plasmid with a mutated Ad1 showed no response to cAMP. The effect of the mutation at the Ad1 site on the cAMP response was almost the same as that of the deletion of Ad3 and Ad4, although the role of each element seemed to be different. These results indicated that both the Ad1 site and the upstream elements, Ad3 and Ad4, were necessary for the full response to cAMP of the CYP11B gene. When the Ad1 site in the promoter region was replaced with a palindromic CRE, elevated transcription activity was detected both in vitro and in vivo. Two kinds of CREBs (43 and 47 kDa) purified from a HeLa cell nuclear extract bound to the Ad1 site. The binding of the palindromic CRE to the nuclear factor(s) was stronger than that of Ad1.

Cooperative transcription activation between Ad1, a CRE-like element, and other elements in the CYP11B gene promoter. Publishing Authors By Initials

T Hashimoto,K Morohashi,K Takayama,S Honda,T Wada,H Handa,T Omura,

For similar genetic processes: gene expression: transcription, genetic research abstracts see: genetic processes: gene expression: transcription, genetic research

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Cooperative transcription activation between Ad1, a CRE-like element, and other elements in the CYP11B gene promoter. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 112

Page Numbers: 573-5

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 1992

Cooperative transcription activation between Ad1, a CRE-like element, and other elements in the CYP11B gene promoter. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Cooperative transcription activation between Ad1, a CRE-like element, and other elements in the CYP11B gene promoter. Keywords Mesh Terms:

KEYWORDS: Transcription, Genetic

MESH TERMS: genetics

Chemical & Substance for Abstract: Cooperative transcription activation between Ad1, a CRE-like element, and other elements in the CYP11B gene promoter. Information

Substance Name: Steroid 11-beta-Hydroxylase

Registry Number: EC 1.14.15.4

Grant and Affiliation Information for Cooperative transcription activation between Ad1, a CRE-like element, and other elements in the CYP11B gene promoter.

AFFILIATION: Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka.

Country: JAPAN

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MEDLINETA: J Biochem

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