Cooperative binding of an Ultrabithorax homeodomain protein to nearby and distant DNA sites.

Cooperative binding of an Ultrabithorax homeodomain protein to nearby and distant DNA sites. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Cooperative binding of an Ultrabithorax homeodomain protein to nearby and distant DNA sites. Abstract Text:

Cooperativity in binding of regulatory proteins to multiple DNA sites can heighten the sensitivity and specificity of the transcriptional response. We report here the cooperative DNA-binding properties of a developmentally active regulatory protein encoded by the Drosophila homeotic gene Ultrabithorax (Ubx). We show that naturally occurring binding sites for the Ubx-encoded protein contain clusters of multiple individual binding site sequences. Such sites can form complexes containing a dozen or more Ubx-encoded protein molecules, with simultaneous cooperative interactions between adjacent and distant DNA sites. The distant mode of interaction involves a DNA looping mechanism; both modes appear to enhance transcriptional activation in a simple yeast assay system. We found that cooperative binding is dependent on sequences outside the homeodomain, and we have identified regions predicted to form coiled coils carboxy terminal to the homeodomains of the Ubx-encoded protein and several other homeotic proteins. On the basis of our findings, we propose a multisite integrative model of homeotic protein action in which functional regulatory elements can be built from a few high-affinity sites, from many lower-affinity sites, or from sites of some intermediate number and affinity. An important corollary of this model is that even small differences in binding of homeotic proteins to individual sites could be summed to yield large overall differences in binding to multiple sites. This model is consistent with reports that homeodomain protein targets contain multiple individual binding site sequences distributed throughout sizable DNA regions. Also consistent is a recent report that sequences carboxy terminal to the Ubx homeodomain can contribute to segmental specificity.

Cooperative binding of an Ultrabithorax homeodomain protein to nearby and distant DNA sites. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Cooperative binding of an Ultrabithorax homeodomain protein to nearby and distant DNA sites. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular and cellular biology

VOLUME: 13

Page Numbers: 6941-56

Journal Abbreviation: Mol. Cell. Biol.

ISSN: 0270-7306

DAY: 15

MONTH: Nov

YEAR: 1993

Cooperative binding of an Ultrabithorax homeodomain protein to nearby and distant DNA sites. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8109087

Cooperative binding of an Ultrabithorax homeodomain protein to nearby and distant DNA sites. Keywords Mesh Terms:

KEYWORDS: Transcription, Genetic

MESH TERMS: ultrastructure

Chemical & Substance for Abstract: Cooperative binding of an Ultrabithorax homeodomain protein to nearby and distant DNA sites. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Cooperative binding of an Ultrabithorax homeodomain protein to nearby and distant DNA sites.

AFFILIATION: Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.

Country: UNITED STATES

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Mol Cell Biol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Cooperative binding of an Ultrabithorax homeodomain protein to nearby and distant DNA sites Related Publications

• A PATO-compliant zebrafish screening database (MODB): management of morpholino knockdown screen information.
• A facile method for somatic, lifelong manipulation of multiple genes in the mouse liver.
• Cooperative binding of an Ultrabithorax homeodomain protein to nearby and distant DNA sites.
• Distinct cis-regulatory elements from the Dlx1/Dlx2 locus mark different progenitor cell populations in the ganglionic eminences and different subtypes of adult cortical interneurons.
• Down on the (fish) farm.
• Expression and regulation of mouse Mtsh1 during limb and branchial arch development.
• Functional resolution of duplicated hoxb5 genes in teleosts.
• Gene-breaking transposon mutagenesis reveals an essential role for histone H2afza in zebrafish larval development.
• Harnessing a high cargo-capacity transposon for genetic applications in vertebrates.
• Insertional mutagenesis strategies in zebrafish.
• Looking to the future.
• Modulation of gene expression in the study of zebrafish development-a discussion with james chen, ph.d.
• The three musketeers of HSC development.
• Trapping fish genes with transposons.
• Zebrafish as a genomics research model.
• Zebrafish dlx2a contributes to hindbrain neural crest survival, is necessary for differentiation of sensory ganglia and functions with dlx1a in maturation of the arch cartilage elements.
• Zinc finger-based knockout punches for zebrafish genes.
• p53 activation by knockdown technologies.