Convenient transformation of anamorphic basidiomycetous yeasts belonging to genus pseudozyma induced by electroporation.

Convenient transformation of anamorphic basidiomycetous yeasts belonging to genus pseudozyma induced by electroporation. Research Abstract Details 

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Convenient transformation of anamorphic basidiomycetous yeasts belonging to genus pseudozyma induced by electroporation. Abstract Text:

Tomotake Morita,Hiroshi Habe,Tokuma Fukuoka,Tomohiro Imura,Dai Kitamoto,Tomotake Morita,Hiroshi Habe,Tokuma Fukuoka,Tomohiro Imura,Dai Kitamoto,Tomotake Morita,Hiroshi Habe,Tokuma Fukuoka,Tomohiro Imura,Dai Kitamoto,

A convenient procedure for carrying out transformation by electroporation was optimized for the genus Pseudozyma. Successful transformation was achieved using a plasmid, pUXV1, that confers resistance to hygromycin B; the maximum transformation efficiency was 48 transformants/mug of plasmid DNA. Transformants of Pseudozyma antarctica T-34 expressing a green fluorescent protein were obtained by the procedure.

Convenient transformation of anamorphic basidiomycetous yeasts belonging to genus pseudozyma induced by electroporation. Publishing Authors By Initials

T Morita,H Habe,T Fukuoka,T Imura,D Kitamoto,T Morita,H Habe,T Fukuoka,T Imura,D Kitamoto,T Morita,H Habe,T Fukuoka,T Imura,D Kitamoto,

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Convenient transformation of anamorphic basidiomycetous yeasts belonging to genus pseudozyma induced by electroporation. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of bioscience and bioengineering

VOLUME: 104

Page Numbers: 517-20

Journal Abbreviation: J. Biosci. Bioeng.

ISSN: 1389-1723

DAY: 24

MONTH: Dec

YEAR: 2007

Convenient transformation of anamorphic basidiomycetous yeasts belonging to genus pseudozyma induced by electroporation. Information

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LANGUAGE: eng

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AFFILIATION: Research Institute for Innovations in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, 1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.

Country: Japan

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MEDLINETA: J Biosci Bioeng

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