Controlling data quality and reproducibility of a high-sensitivity immunoassay using isotachophoresis in a microchip.

Controlling data quality and reproducibility of a high-sensitivity immunoassay using isotachophoresis in a microchip. Research Abstract Details 

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Controlling data quality and reproducibility of a high-sensitivity immunoassay using isotachophoresis in a microchip. Abstract Text:

C Charles Park,Irina Kazakova,Tomohisa Kawabata,Michael Spaid,Ring-Ling Chien,H Garrett Wada,Shinji Satomura,

This report describes a method of controlling the sensitivity and reproducibility of a microchip-based immunoassay by using isotachophoresis to preconcentrate the antigen and antibody prior to binding. Gel electrophoresis separation is coupled to the preconcentration step to separate the immunocomplex products formed. The system employs a quartz-based LabChip that automates the metering, preconcentration, reaction, separation, and detection. The system also uses a handoff mechanism that switches the immunocomplex from the stacking mode to the separation mode. We show that the handoff timing affects the data quality and repeatability of the electropherograms, and we demonstrate an automatic handoff mechanism to precisely control the signal intensity and separation of peaks of interest. In so doing, the automatic handoff mechanism also improves the reproducibility of the assay. When applied to the homogeneous liquid-phase detection of alpha-fetoprotein, a common tumor marker, the system shows a greater than 200-fold stacking of specific analytes of interest.

Controlling data quality and reproducibility of a high-sensitivity immunoassay using isotachophoresis in a microchip. Publishing Authors By Initials

CC Park,I Kazakova,T Kawabata,M Spaid,RL Chien,HG Wada,S Satomura,

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Controlling data quality and reproducibility of a high-sensitivity immunoassay using isotachophoresis in a microchip. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Analytical chemistry

VOLUME: 80

Page Numbers: 808-14

Journal Abbreviation: Anal. Chem.

ISSN: 0003-2700

DAY: 4

MONTH: 01

YEAR: 2008

Controlling data quality and reproducibility of a high-sensitivity immunoassay using isotachophoresis in a microchip. Information

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LANGUAGE: eng

NlmUniqueID: 370536

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Grant and Affiliation Information for Controlling data quality and reproducibility of a high-sensitivity immunoassay using isotachophoresis in a microchip.

AFFILIATION: Caliper Life Sciences, Mountain View, California 94043, Wako Pure Chemical Industries, Mountain View, California 94043, and Cambrios Technologies, Mountain View, California 94043.

Country: United States

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MEDLINETA: Anal Chem

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