Controlled microaspiration for high-pressure freezing: a new method for ultrastructural preservation of fragile and sparse tissues for TEM and electron tomography.

Controlled microaspiration for high-pressure freezing: a new method for ultrastructural preservation of fragile and sparse tissues for TEM and electron tomography. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Controlled microaspiration for high-pressure freezing: a new method for ultrastructural preservation of fragile and sparse tissues for TEM and electron tomography. Abstract Text:

High-pressure freezing is the preferred method to prepare thick biological specimens for ultrastructural studies. However, the advantages obtained by this method often prove unattainable for samples that are difficult to handle during the freezing and substitution protocols. Delicate and sparse samples are difficult to manipulate and maintain intact throughout the sequence of freezing, infiltration, embedding and final orientation for sectioning and subsequent transmission electron microscopy. An established approach to surmount these difficulties is the use of cellulose microdialysis tubing to transport the sample. With an inner diameter of 200 mum, the tubing protects small and fragile samples within the thickness constraints of high-pressure freezing, and the tube ends can be sealed to avoid loss of sample. Importantly, the transparency of the tubing allows optical study of the specimen at different steps in the process. Here, we describe the use of a micromanipulator and microinjection apparatus to handle and position delicate specimens within the tubing. We report two biologically significant examples that benefit from this approach, 3D cultures of mammary epithelial cells and cochlear outer hair cells. We illustrate the potential for correlative light and electron microscopy as well as electron tomography.

Controlled microaspiration for high-pressure freezing: a new method for ultrastructural preservation of fragile and sparse tissues for TEM and electron tomography. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Controlled microaspiration for high-pressure freezing: a new method for ultrastructural preservation of fragile and sparse tissues for TEM and electron tomography. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of microscopy

VOLUME: 230

Page Numbers: 278-87

Journal Abbreviation:

ISSN: 1365-2818

DAY: 30

MONTH: May

YEAR: 2008

Controlled microaspiration for high-pressure freezing: a new method for ultrastructural preservation of fragile and sparse tissues for TEM and electron tomography. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 204522

Controlled microaspiration for high-pressure freezing: a new method for ultrastructural preservation of fragile and sparse tissues for TEM and electron tomography. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Controlled microaspiration for high-pressure freezing: a new method for ultrastructural preservation of fragile and sparse tissues for TEM and electron tomography. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Controlled microaspiration for high-pressure freezing: a new method for ultrastructural preservation of fragile and sparse tissues for TEM and electron tomography.

AFFILIATION: Life Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Mail Stop Donner, Berkeley, CA 94720, U.S.A., and Department of Bioengineering, Rice University, MS 142, P.O. Box 1892, Houston, TX 77251-1892, U.S.A.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Microsc

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Controlled microaspiration for high-pressure freezing: a new method for ultrastructural preservation of fragile and sparse tissues for TEM and electron tomography Related Publications

• Beyond dimerization: a membrane-dependent activation model for interleukin-4 receptor-mediated signalling.
• Brain white matter hyperintensities are associated with carotid intraplaque hemorrhage.
• Chronic progressive external ophthalmoplegia: MR spectroscopy and MR diffusion studies in the brain.
• Commentary.
• Comparison of computer assisted surgery with conventional technique for treatment of abaxial distal phalanx fractures in horses: an in vitro study.
• Controlled microaspiration for high-pressure freezing: a new method for ultrastructural preservation of fragile and sparse tissues for TEM and electron tomography.
• Covalent fluorescence labeling of his-tagged proteins on the surface of living cells.
• Crystal structures of the chiral lithiosilanes [(Lis)-PhMe2SiLi x THF x (-)-sparteine] and [Ph2(NEt2)SiLi x (-)-sparteine].
• Development of a new PCR protocol for the detection of species and genotypes (strains) of Echinococcus in formalin-fixed, paraffin-embedded tissues.
• Diseases of the carpus.
• Effects of altered corticosteroid milieu on rat hippocampal neurochemistry and structure - An in vivo magnetic resonance spectroscopy and imaging study.
• Electron tomography: a 3D view of the subcellular world.
• Fracture configurations of the equine radius and tibia after a simulated kick.
• Genetic alteration associated with chronic lymphocytic leukemia.
• Intraoperative ultrasound as guide for neurosurgical endoscopic procedures.
• Intraplaque haemorrhage mimicking carotid pseudoaneurysm on magnetic resonance angiography.
• Is somatization a habituation disorder? Physiological reactivity in somatization syndrome.
• Lung cancer histogenesis following in vivo bronchial injections of 20-methylcholanthrene in dogs.
• Mammary carcinoma: DNA analysis in areas showing different histological features in the same tumor.
• Mammary carcinoma: comparison of DNA-content in the primary tumor and the corresponding axillary lymphnode metastases.
• N(pro) fusion technology to produce proteins with authentic N termini in E. coli.
• Neurotoxocarosis.
• Reproducibility of the cellular DNA-distribution patterns in multiple fine needle aspirates from human malignant tumors.
• Rheumatic fever and the blood groups.
• Role of subvoxel free fluid on diffusion parameters in brain tissue with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy and its correlation with physical disability: histogram analysis of standard and fluid-atte
• Serial proton MR spectroscopy and diffusion imaging findings in HIV-related herpes simplex encephalitis.
• Single-bead, Single-molecule, Single-cell Fluorescence: Technologies for Drug Screening and Target Validation.
• Syncope and trauma. Are syncope-related traumatic injuries the key to find the specific cause of the symptom?
• Truncation of the krebs cycle during hypoglycemic coma.
• [Immune reactions in gastrointestinal diseases with autoimmune markers]