Control of gene conversion and somatic hypermutation by immunoglobulin promoter and enhancer sequences.

Control of gene conversion and somatic hypermutation by immunoglobulin promoter and enhancer sequences. Research Abstract Details 

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Control of gene conversion and somatic hypermutation by immunoglobulin promoter and enhancer sequences. Abstract Text:

Shu Yuan Yang,Sebastian D Fugmann,David G Schatz,

It is thought that gene conversion (GCV) and somatic hypermutation (SHM) of immunoglobulin (Ig) genes occur in two steps: the generation of uracils in DNA by activation-induced cytidine deaminase, followed by their subsequent repair by various DNA repair pathways to generate sequence-diversified products. It is not known how either of the two steps is targeted specifically to Ig loci. Because of the tight link between transcription and SHM, we have investigated the role of endogenous Ig light chain (IgL) transcriptional control elements in GCV/SHM in the chicken B cell line DT40. Promoter substitution experiments led to identification of a strong RNA polymerase II promoter incapable of supporting efficient GCV/SHM. This surprising finding indicates that high levels of transcription are not sufficient for robust GCV/SHM in Ig loci. Deletion of the IgL enhancer in a context in which high-level transcription was not compromised showed that the enhancer is not necessary for GCV/SHM. Our results indicate that cis-acting elements are important for Ig gene diversification, and we propose that targeting specificity is achieved through the combined action of several Ig locus elements that include the promoter.

Control of gene conversion and somatic hypermutation by immunoglobulin promoter and enhancer sequences. Publishing Authors By Initials

SY Yang,SD Fugmann,DG Schatz,

For similar investigative techniques: genetic techniques: gene transfer techniques: transfection research abstracts see: investigative techniques: genetic techniques: gene transfer techniques: transfection research

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Control of gene conversion and somatic hypermutation by immunoglobulin promoter and enhancer sequences. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of experimental medicine

VOLUME: 203

Page Numbers: 2919-28

Journal Abbreviation: J. Exp. Med.

ISSN: 0022-1007

DAY: 18

MONTH: 12

YEAR: 2006

Control of gene conversion and somatic hypermutation by immunoglobulin promoter and enhancer sequences. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985109

Control of gene conversion and somatic hypermutation by immunoglobulin promoter and enhancer sequences. Keywords Mesh Terms:

KEYWORDS: Transfection

MESH TERMS: genetics

Chemical & Substance for Abstract: Control of gene conversion and somatic hypermutation by immunoglobulin promoter and enhancer sequences. Information

Substance Name: Cytidine Deaminase

Registry Number: EC 3.5.4.5

Grant and Affiliation Information for Control of gene conversion and somatic hypermutation by immunoglobulin promoter and enhancer sequences.

AFFILIATION: Section of Immunobiology and Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06520, USA.

Country: United States

AGENCY: United States NIAID

GRANT: AI066130

ACRONYM: AI

MEDLINETA: J Exp Med

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