[Control genes of chondrocyte apoptosis in osteoarthritic articular cartilage]

[Control genes of chondrocyte apoptosis in osteoarthritic articular cartilage] Research Abstract Details 

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[Control genes of chondrocyte apoptosis in osteoarthritic articular cartilage] Abstract Text:

J Hu,G Huang,S Huang,L Yang,

OBJECTIVE: To investigate the expression of bax and bcl-2 in normal human articular chondrocytes and in osteoarthritic articular cartilage. METHODS: The samples of articular cartilage were obtained from 9 patients and 6 normal subjects. Bax and bcl-2 mRNA were detected by reverse transcriptase/polymerase chain reaction (RT-PCR) and their expression proteins were analyzed immunohistochemically. TUNEL technique was used to study apoptosis in situ. RESULTS: Bax and bcl-2 mRNA was detectable in chondrocytes of both osteoarthritic and normal cartilage. Bax mRNA of chondrocytes from patients with osteoarthritis (OA) was overexpressed compared with the normal controls (P < 0.01), and OA cartilage chondrocyte also expressed more bcl-2 mRNA than the controls (P < 0.05). There was no statistically significant difference of bax/bcl-2 between the two groups. Immunohistochemical staining demonstrated the same level of bax and bcl-2 proteins as their mRNA. A greater proportion of apoptotic chondrocytes were found in the OA cartilage than that in normal controls (4% - 14% versus 0 - 2%). CONCLUSIONS: Our results suggested that chondrocyte apoptosis was co-regulated by both bax and bcl-2. The ratio of bax to bcl-2 may contribute not only to the lower percentage of apoptotic chondrocytes, but also to the chronic pathologic process in OA.

[Control genes of chondrocyte apoptosis in osteoarthritic articular cartilage] Publishing Authors By Initials

J Hu,G Huang,S Huang,L Yang,

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[Control genes of chondrocyte apoptosis in osteoarthritic articular cartilage] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zhonghua wai ke za zhi [Chinese journal of surgery

VOLUME: 38

Page Numbers: 266-8, 17

Journal Abbreviation: Zhonghua Wai Ke Za Zhi

ISSN: 0529-5815

DAY: 8

MONTH: Apr

YEAR: 2000

[Control genes of chondrocyte apoptosis in osteoarthritic articular cartilage] Information

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LANGUAGE: chi

NlmUniqueID: 153611

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AFFILIATION: Department of Orthopaedic, Beijing Hospital, Beijing 100730, China.

Country: China

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MEDLINETA: Zhonghua Wai Ke Za Zhi

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