Contribution of glutamate transporter GLT-1 to removal of synaptically released glutamate at climbing fiber-Purkinje cell synapses.

Contribution of glutamate transporter GLT-1 to removal of synaptically released glutamate at climbing fiber-Purkinje cell synapses. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Contribution of glutamate transporter GLT-1 to removal of synaptically released glutamate at climbing fiber-Purkinje cell synapses. Abstract Text:

Yusuke Takatsuru,Masae Iino,Kohichi Tanaka,Seiji Ozawa,

Rapid removal of synaptically released glutamate from the extracellular space ensures a high signal-to-noise ratio in excitatory neurotransmission. In the cerebellum, glial glutamate transporters, GLAST and GLT-1, are co-localized in the processes of Bergmann glia wrapping excitatory synapses on Purkinje cells (PCs). Although GLAST is expressed six-fold more abundantly than GLT-1, the decay kinetics of climbing fiber-mediated excitatory postsynaptic currents (CF-EPSCs) in PCs in GLAST(-/-) mice are not different from those in wild-type (WT) mice. This raises a possibility that GLT-1 plays a significant role in clearing glutamate at CF-PC synapses despite its smaller amount of expression. Here, we studied the functions of GLT-1 and GLAST in the clearance of glutamate using GLAST(-/-) mice and GLT-1(-/-) mice. In the presence of cyclothiazide (CTZ) that attenuates the desensitization of AMPA receptors, the decay time constant of CF-EPSCs (tau(w)) in GLT-1(-/-) mice was slower than that in WT mice. However, the degree of this prolongation of tau(w) was less prominent compared to that in GLAST(-/-) mice. The values of tau(w) in GLT-1(-/-) mice and GLAST(-/-) mice were comparable to those estimated in WT mice in the presence of a potent blocker of glial glutamate transporters (2S,3S)-3-[3-(4-methoxybenzoylamino)benzyloxy]aspartate (PMB-TBOA) at 10 and 100nM, which reduced the amplitudes of glutamate transporter currents elicited by CF stimulation in Bergmann glia to approximately 81 and approximately 28%, respectively. We conclude that GLT-1 plays a minor role compared to GLAST in clearing synaptically released glutamate at CF-PC synapses.

Contribution of glutamate transporter GLT-1 to removal of synaptically released glutamate at climbing fiber-Purkinje cell synapses. Publishing Authors By Initials

Y Takatsuru,M Iino,K Tanaka,S Ozawa,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Contribution of glutamate transporter GLT-1 to removal of synaptically released glutamate at climbing fiber-Purkinje cell synapses. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Neuroscience letters

VOLUME: 420

Page Numbers: 85-9

Journal Abbreviation: Neurosci. Lett.

ISSN: 0304-3940

DAY: 3

MONTH: 05

YEAR: 2007

Contribution of glutamate transporter GLT-1 to removal of synaptically released glutamate at climbing fiber-Purkinje cell synapses. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7600130

Contribution of glutamate transporter GLT-1 to removal of synaptically released glutamate at climbing fiber-Purkinje cell synapses. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Contribution of glutamate transporter GLT-1 to removal of synaptically released glutamate at climbing fiber-Purkinje cell synapses. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Contribution of glutamate transporter GLT-1 to removal of synaptically released glutamate at climbing fiber-Purkinje cell synapses.

AFFILIATION: Department of Neurophysiology, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan; Division of Homeostatic Development, National Institute for Physiological Sciences, 38 Nishigonaka Myodaiji, Okazaki, Aichi 444-8585, Japan.

Country: Ireland

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Neurosci Lett

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Contribution of glutamate transporter GLT-1 to removal of synaptically released glutamate at climbing fiber-Purkinje cell synapses Related Publications

• A secure distribution method for digitized image scan using a two-step wavelet-based technique: A Telemedicine Case.
• Acyl-adenylate formation coupled with mitochondrial respiration.
• Adenosine diphosphatase in the electron transfer particle.
• Association of TIMP-2 with extracellular matrix exposed to mechanical stress and its co-distribution with periostin during mouse mandible development.
• Bisphosphonate incadronate inhibits maturation of ectopic bone induced by recombinant human bone morphogenetic protein 2.
• Bridging reagent for protein. I. The reaction of diisocyanates with lysine and enzyme proteins.
• Bridging reagent for protein. II. The reaction of N,N'-polymethylene-bis(iodoacetamide) with cysteine and rabbit muscle aldolase.
• Cerebellar infarction secondary to os odontoideum.
• Comparison of 4 MV photon surface dose among Varian, Siemens, and Elekta linear accelerators for tangential breast treatment: a phantom study.
• Contribution of glutamate transporter GLT-1 to removal of synaptically released glutamate at climbing fiber-Purkinje cell synapses.
• Duplication of the inferior vena cava associated with other variations.
• Esterification of adenosine monophosphate coupled with the respiration of heavy beef heart mitochondria.
• Facilitated activation of metabotropic glutamate receptors in cerebellar Purkinje cells in glutamate transporter EAAT4-deficient mice.
• Fibroblasts of spinal ligaments pathologically differentiate into chondrocytes induced by recombinant human bone morphogenetic protein-2: morphological examinations for ossification of spinal ligaments.
• Fluorescence changes in mitochondrial protein associated with energy state.
• Gene therapy using AAV.
• Leuprorelin acetate for female patients with newly diagnosed acute leukemia receiving chemotherapy.
• Long-term evaluation of recombinant human bone morphogenetic protein-2 induced bone formation with a biologic and synthetic delivery system.
• Macroscopic and histological findings in the healing process of inhalation injury.
• Mechanisms of immunomodulation by mesenchymal stem cells.
• Membrane properties of rat medial vestibular nucleus neurons in vivo.
• Morphological substrate of autonomic failure and neurohormonal dysfunction in multiple system atrophy: impact on determining phenotype spectrum.
• Occurrence of ubiquinone and rhodoquinone in parasitic nematodes, Metastrangylus elongatus and Ascaris lumbricoides var. suis.
• On the isolation of mitochondria with high respiratory control from guinea pig pancreas.
• Phosphorylation of bound adenosine monophosphate in the electron transfer particle.
• Soft X-ray multilayer beam splitters.
• The detection and visualization of brain tumors on T2-weighted MRI images using multiparameter feature blocks.
• The effect of phosphine on respiration of rat liver mitochondria.
• Vector-producing tumor-tracking multipotent mesenchymal stromal cells for suicide cancer gene therapy.
• [Comparative study of drug efficacy and drug additives between generic drugs and original drugs]