Contractility and Ca2+ signaling of smooth muscle cells in different generations of mouse airways.

Contractility and Ca2+ signaling of smooth muscle cells in different generations of mouse airways. Research Abstract Details 

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Contractility and Ca2+ signaling of smooth muscle cells in different generations of mouse airways. Abstract Text:

Yan Bai,Minsi Zhang,Michael J Sanderson,

The control and mechanisms of airway smooth muscle cell (SMC) contraction were investigated with a sequential series of lung slices from different generations of the same airway from the cardiac lobe of the mouse lung. Airway contraction was measured by monitoring the changes in airway lumen area with phase-contrast microscopy. Changes in intracellular calcium concentration of the SMCs were studied with a custom-built confocal or two-photon microscope. The distribution of the airway SMCs and the muscarinic M(3) or 5-HT(2A) receptors was determined with immunofluorescence. Methacholine and 5-HT induced a concentration-dependent airway contraction and Ca(2+) oscillations within the SMCs of each airway generation. The airway contraction in response to the same agonist concentration was greater in the middle generation compared with the distal or proximal generations of the same airway. Similarly, the Ca(2+) oscillations varied in different generations of the same airway, with a slower frequency in the SMCs of the distal zone as compared with the middle or proximal zones of airways. By contrast, high KCl induced minimal contraction and very slow Ca(2+) oscillations throughout the whole intrapulmonary airway. The slower agonist-induced Ca(2+) oscillations in the distal zone correlated with a reduced expression of agonist receptors. The layer of SMCs increased in thickness in the middle and proximal zones. These results indicate that the contractility of airway SMCs varies at different positions along the same airway and that this response partially results from different Ca(2+) signaling and the total amount of the SMCs.

Contractility and Ca2+ signaling of smooth muscle cells in different generations of mouse airways. Publishing Authors By Initials

Y Bai,M Zhang,MJ Sanderson,

For similar organic chemicals: amines: biogenic amines: biogenic monoamines: tryptamines: serotonin research abstracts see: organic chemicals: amines: biogenic amines: biogenic monoamines: tryptamines: serotonin research

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MEDLINE DATE:

Contractility and Ca2+ signaling of smooth muscle cells in different generations of mouse airways. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: American journal of respiratory cell and molecular

VOLUME: 36

Page Numbers: 122-30

Journal Abbreviation: Am. J. Respir. Cell Mol. Biol.

ISSN: 1044-1549

DAY: 24

MONTH: 08

YEAR: 2006

Contractility and Ca2+ signaling of smooth muscle cells in different generations of mouse airways. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8917225

Contractility and Ca2+ signaling of smooth muscle cells in different generations of mouse airways. Keywords Mesh Terms:

KEYWORDS: Serotonin

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Contractility and Ca2+ signaling of smooth muscle cells in different generations of mouse airways. Information

Substance Name: Potassium Chloride

Registry Number: 7447-40-7

Grant and Affiliation Information for Contractility and Ca2+ signaling of smooth muscle cells in different generations of mouse airways.

AFFILIATION: Department of Physiology, University of Massachusetts Medical School, Worcester, MA 01655, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: HL 71930

ACRONYM: HL

MEDLINETA: Am J Respir Cell Mol Biol

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