Context based mixture model for cell phase identification in automated fluorescence microscopy.

Context based mixture model for cell phase identification in automated fluorescence microscopy. Research Abstract Details 

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Context based mixture model for cell phase identification in automated fluorescence microscopy. Abstract Text:

Meng Wang,Xiaobo Zhou,Randy W King,Stephen T C Wong,

BACKGROUND: Automated identification of cell cycle phases of individual live cells in a large population captured via automated fluorescence microscopy technique is important for cancer drug discovery and cell cycle studies. Time-lapse fluorescence microscopy images provide an important method to study the cell cycle process under different conditions of perturbation. Existing methods are limited in dealing with such time-lapse data sets while manual analysis is not feasible. This paper presents statistical data analysis and statistical pattern recognition to perform this task. RESULTS: The data is generated from Hela H2B GFP cells imaged during a 2-day period with images acquired 15 minutes apart using an automated time-lapse fluorescence microscopy. The patterns are described with four kinds of features, including twelve general features, Haralick texture features, Zernike moment features, and wavelet features. To generate a new set of features with more discriminate power, the commonly used feature reduction techniques are used, which include Principle Component Analysis (PCA), Linear Discriminant Analysis (LDA), Maximum Margin Criterion (MMC), Stepwise Discriminate Analysis based Feature Selection (SDAFS), and Genetic Algorithm based Feature Selection (GAFS). Then, we propose a Context Based Mixture Model (CBMM) for dealing with the time-series cell sequence information and compare it to other traditional classifiers: Support Vector Machine (SVM), Neural Network (NN), and K-Nearest Neighbor (KNN). Being a standard practice in machine learning, we systematically compare the performance of a number of common feature reduction techniques and classifiers to select an optimal combination of a feature reduction technique and a classifier. A cellular database containing 100 manually labelled subsequence is built for evaluating the performance of the classifiers. The generalization error is estimated using the cross validation technique. The experimental results show that CBMM outperforms all other classifies in identifying prophase and has the best overall performance. CONCLUSION: The application of feature reduction techniques can improve the prediction accuracy significantly. CBMM can effectively utilize the contextual information and has the best overall performance when combined with any of the previously mentioned feature reduction techniques.

Context based mixture model for cell phase identification in automated fluorescence microscopy. Publishing Authors By Initials

M Wang,X Zhou,RW King,ST Wong,

For similar information science: pattern recognition, automated research abstracts see: information science: pattern recognition, automated research

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Context based mixture model for cell phase identification in automated fluorescence microscopy. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: BMC bioinformatics

VOLUME: 8

Page Numbers: 32

Journal Abbreviation: BMC Bioinformatics

ISSN: 1471-2105

DAY: 30

MONTH: 01

YEAR: 2007

Context based mixture model for cell phase identification in automated fluorescence microscopy. Information

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LANGUAGE: eng

NlmUniqueID: 100965194

Context based mixture model for cell phase identification in automated fluorescence microscopy. Keywords Mesh Terms:

KEYWORDS: Pattern Recognition, Automated

MESH TERMS: methods

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Grant and Affiliation Information for Context based mixture model for cell phase identification in automated fluorescence microscopy.

AFFILIATION: Center for Bioinformatics, Harvard Center for Neurodegeneration and Repair, Harvard Medical School, 3rd floor, 1249 Boylston, Boston, MA 02215, USA. bioinformaticswang@gmail.com <bioinformaticswang@gmail.com>

Country: England

AGENCY: United States NLM

GRANT: R01 LM008696

ACRONYM: LM

MEDLINETA: BMC Bioinformatics

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