Construction of YAC libraries with large inserts.

Construction of YAC libraries with large inserts. Research Abstract Details 

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Construction of YAC libraries with large inserts. Abstract Text:

The yeast artificial chromosome (YAC) cloning system makes it possible to clone large pieces of genomic DNA into yeast. Libraries have been made containing clones with inserts in the megabase-pair range. The basic protocol in this unit describes preparation of YAC vectors and transformation of ligated DNA into yeast spheroplasts. A support protocol describes titration of Lyticase to make spheroplasts. Additional support protocols detail two methods for partial digestion of genomic DNA: EcoRI restriction endonuclease-EcoRI methylase competition and the partial digestion of genomic DNA by use of limiting amounts of Mg2+, respectively.

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Construction of YAC libraries with large inserts. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Current protocols in human genetics / editorial bo

VOLUME: Chapter 5

Page Numbers: Unit5.2

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ISSN: 1934-8258

DAY: 22

MONTH: Feb

YEAR: 2002

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LANGUAGE: eng

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AFFILIATION: Whitehead Institute, Cambridge, Massachusetts, USA.

Country: United States

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MEDLINETA: Curr Protoc Hum Genet

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