[Construction of VEGF eukaryotic expression vector and its expression in endothelial cells and cardiac myocytes in-vitro]

[Construction of VEGF eukaryotic expression vector and its expression in endothelial cells and cardiac myocytes in-vitro] Research Abstract Details 

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[Construction of VEGF eukaryotic expression vector and its expression in endothelial cells and cardiac myocytes in-vitro] Abstract Text:

Ming Yu,Yun-Qiu Qian,Shao-Ying Lu,Guang-Sheng Chen,Jing Ye,

AIM:To study the feasibility of VEGF165 gene transfection to endothelial cells and cardiac myocytes in-vitro. METHODS: The VEGF165 DNA fragment was inserted into a green fluorescent protein fusion vector, which was then transfected into endothelial cells and cardiac myocytes with lipofectamine. RESULTS: The endothelial cells and cardiac myocytes were successfully transfected with pIRES2-EGFP-hVEGF165 gene, which was confirmed by fluoroscopy and immunohistochemistry. CONCLUSION: VEGF can be expressed stably in the endothelial cells and cardiac myocytes, which might be useful for the gene therapy of myocardial ischemia.

[Construction of VEGF eukaryotic expression vector and its expression in endothelial cells and cardiac myocytes in-vitro] Publishing Authors By Initials

M Yu,YQ Qian,SY Lu,GS Chen,J Ye,

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[Construction of VEGF eukaryotic expression vector and its expression in endothelial cells and cardiac myocytes in-vitro] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese jour

VOLUME: 19

Page Numbers: 223-4

Journal Abbreviation: Xi Bao Yu Fen Zi Mian Yi Xue Z

ISSN: 1007-8738

DAY: 24

MONTH: May

YEAR: 2003

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LANGUAGE: chi

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AFFILIATION: Department of Ultrasound, Xijing Hospital, Fourth Military Medical University, Xi'an 710032,China.

Country: China

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MEDLINETA: Xi Bao Yu Fen Zi Mian Yi Xue Z

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