[Construction of the prokaryotic expression vector of MTB lhp gene and its expression]

[Construction of the prokaryotic expression vector of MTB lhp gene and its expression] Research Abstract Details 

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[Construction of the prokaryotic expression vector of MTB lhp gene and its expression] Abstract Text:

Quan Chen,Xu-Dong Luo,Yin Jiang,Shan Jiang,Dao-Yin Zhu,

AIM:To construct prokaryotic expression vector carrying lhp gene and express it in E.coli. METHODS: The MTB lhp gene was amplified by PCR and then cloned into plasmid pQE30. After sequencing, the gene was cloned into plasmid pET32a(+) to construct recombinant prokaryotic expression vectors pQE30-CFP10 and pET32a(+)-CFP10. RESULTS: After transformation of the E.coli and induction with 1 mmol/L of IPTG, no additional protein was expressed in pQE30-CFP10 system, but recombinant target protein with M(r) 20 000 or so was expressed in pET32a(+)-CFP10 system, and the expressed protein was maximum when induced with IPTG for 4 h. The expressed protein existed in cytoplasm in soluble form and amounted to 38% of total protein of E.coli. Western blot analysis showed that the protein had good antigenicity. The purity of the protein purified through the Ni-NTA resin reached 93%. CONCLUSION: The prokaryotic expression vector pET32a(+)-CFP10 was constructed successfully and the rCFP10 protein was obtained, which laid the foundation for application of the rCFP10.

[Construction of the prokaryotic expression vector of MTB lhp gene and its expression] Publishing Authors By Initials

Q Chen,XD Luo,Y Jiang,S Jiang,DY Zhu,

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[Construction of the prokaryotic expression vector of MTB lhp gene and its expression] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese jour

VOLUME: 19

Page Numbers: 332-4

Journal Abbreviation: Xi Bao Yu Fen Zi Mian Yi Xue Z

ISSN: 1007-8738

DAY: 27

MONTH: Jul

YEAR: 2003

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LANGUAGE: chi

NlmUniqueID: 101139110

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Grant and Affiliation Information for [Construction of the prokaryotic expression vector of MTB lhp gene and its expression]

AFFILIATION: Department of Microbiology, Chongqing University of Medical Science, Chongqing 400016,China.

Country: China

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MEDLINETA: Xi Bao Yu Fen Zi Mian Yi Xue Z

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