[Construction of pcDNA3.1AM and expression of adrenomedullin in mammalian cells]

[Construction of pcDNA3.1AM and expression of adrenomedullin in mammalian cells] Research Abstract Details 

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[Construction of pcDNA3.1AM and expression of adrenomedullin in mammalian cells] Abstract Text:

Xiao-Fang Wang,Ying Shao,De-Zhi Tian,Tai Yao,Li-Min Lu,

The newly discovered endogenous vasodilating and diuretic peptide adrenomedullin (AM) was considered to be of attractive value in clinical treatment of hypertension and congestive heart failure. In order to explore the treatment of cardiovascular diseases by expressing AM in vivo, AM cDNA was inserted into mammalian expressing vector pcDNA3.1, and in vitro expression of AM was carried out in cultured K(562) cell line. AM mRNA was amplified by RT-PCR from the total RNA isolated from the adrenal glands of rats and was inserted into pcDNA3.1 vector to form pcDNA3.1AM, the recombinant pcDNA3.1AM was then transferred into cultured K(562) cell line by liposome. The expression of AM in pcDNA3.1AM transferred cell was identified by RT-PCR and dot immunoblot assay. The results demonstrated that there were AM mRNA in the pcDNA3.1AM-transferred K(562) cell line and AM peptides in the culturing medium, indicating that the recombinant pcDNA3.1AM vector can express AM in mammalian cell line.

[Construction of pcDNA3.1AM and expression of adrenomedullin in mammalian cells] Publishing Authors By Initials

XF Wang,Y Shao,DZ Tian,T Yao,LM Lu,

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[Construction of pcDNA3.1AM and expression of adrenomedullin in mammalian cells] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Sheng li xue bao : [Acta physiologica Sinica]

VOLUME: 55

Page Numbers: 71-4

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ISSN: 0371-0874

DAY: 25

MONTH: Feb

YEAR: 2003

[Construction of pcDNA3.1AM and expression of adrenomedullin in mammalian cells] Information

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LANGUAGE: chi

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Grant and Affiliation Information for [Construction of pcDNA3.1AM and expression of adrenomedullin in mammalian cells]

AFFILIATION: Department of Physiology and Pathaphysiology, Shanghai Medical College, Fudan University, Shanghai 200032; E-mail: lmlu@shmu.edu.cn

Country: China

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MEDLINETA: Sheng Li Xue Bao

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