[Construction of eukaryotic expression vector of short hairpin RNA for transforming growth factor-beta1]

[Construction of eukaryotic expression vector of short hairpin RNA for transforming growth factor-beta1] Research Abstract Details 

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[Construction of eukaryotic expression vector of short hairpin RNA for transforming growth factor-beta1] Abstract Text:

Jing Wang,Jun-Zheng Wu,Fu-Ping Guo,Xiu-Li Zhu,De-Sheng Wen,

OBJECTIVE: To construct the plasmid containing short hairpin RNA (shRNA) of TGF-beta1 expression vector. METHODS: Short chain oligonucleotide was designed according to the TGF-beta1 mRNA sequence provided by Genebank, then DNA segment was gained through annealing after chemosynthesis, and then was cloned to pWH1 vector. The recombinant TGF-beta1 shRNA expression vector was evaluated by using enzyme cutting. At last, the constructed TGF-beta1 expression vector was transfected into salivary gland mucoepidermoid carcinoma (Ms) cells by Lipofectomine TM 2000, and its effect on TGF-beta1 expression was observed by RT-PCR and immunohistochemistry. RESULTS: Successful construction was identified by enzyme cutting and the constructed plasmid was called pWH1-TGF-beta1. The shRNA and it inhibited the TGF-beta1 mRNA and protein expression effectively. CONCLUSION: The constructed TGF-beta1 shRNA expression vector can block the TGF-beta1 expression in salivary gland mucoepidermoid carcinoma cells.

[Construction of eukaryotic expression vector of short hairpin RNA for transforming growth factor-beta1] Publishing Authors By Initials

J Wang,JZ Wu,FP Guo,XL Zhu,DS Wen,

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[Construction of eukaryotic expression vector of short hairpin RNA for transforming growth factor-beta1] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yi

VOLUME: 24

Page Numbers: 113-6

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ISSN: 1000-1182

DAY: 17

MONTH: Apr

YEAR: 2006

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LANGUAGE: chi

NlmUniqueID: 9422648

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AFFILIATION: Dept. of Biology, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.

Country: China

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MEDLINETA: Hua Xi Kou Qiang Yi Xue Za Zhi

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