Construction of conjugative gene transfer system between E. coli and moderately thermophilic, extremely acidophilic Acidithiobacillus caldus MTH-04.

Construction of conjugative gene transfer system between E. coli and moderately thermophilic, extremely acidophilic Acidithiobacillus caldus MTH-04. Research Abstract Details 

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Construction of conjugative gene transfer system between E. coli and moderately thermophilic, extremely acidophilic Acidithiobacillus caldus MTH-04. Abstract Text:

Xiangmei Liu,Jianqun Lin,Zheng Zhang,Jiang Bian,Qing Zhao,Ying Liu,Jianqiang Lin,Wangming Yan,Xiangmei Liu,Jianqun Lin,Zheng Zhang,Jiang Bian,Qing Zhao,Ying Liu,Jianqiang Lin,Wangming Yan,

A genetic transfer system for introducing foreign genes to biomining microorganisms is urgently needed. Thus, a conjugative gene transfer system was investigated for a moderately thermophilic, extremely acidophilic biomining bacterium, Acidithiobacillus caldus MTH-04. The broad-host-range IncP plasmids RP4 and R68.45 were transferred directly into A. caldus MTH-04 from Escherichia coli by conjugation at relatively high frequencies. Additionally the broad-host-range IncQ plasmids pJRD215, pVLT33, and pVLT35 were also transferred into A. caldus MTH-04 with the help of plasmid RP4 or strains with plasmid RP4 integrated into their chromosome, such as E. coli SM10. The Km(r) and Sm(r) selectable markers from these plasmids were successfully expressed in A. caldus MTH-04. Futhermore, the IncP and IncQ plasmids were transferred back into E. coli cells from A. caldus MTH-04, thereby confirming the initial transfer of these plasmids from E. coli to A. caldus MTH-04. All the IncP and IncQ plasmids studied were stable in A. caldus MTH-04. Consequently, this development of a conjugational system for A. caldus MTH-04 will greatly facilitate its genetic study.

Construction of conjugative gene transfer system between E. coli and moderately thermophilic, extremely acidophilic Acidithiobacillus caldus MTH-04. Publishing Authors By Initials

X Liu,J Lin,Z Zhang,J Bian,Q Zhao,Y Liu,J Lin,W Yan,X Liu,J Lin,Z Zhang,J Bian,Q Zhao,Y Liu,J Lin,W Yan,

For similar genetic structures: plasmids research abstracts see: genetic structures: plasmids research

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Construction of conjugative gene transfer system between E. coli and moderately thermophilic, extremely acidophilic Acidithiobacillus caldus MTH-04. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of microbiology and biotechnology

VOLUME: 17

Page Numbers: 162-7

Journal Abbreviation: J. Microbiol. Biotechnol.

ISSN: 1017-7825

DAY: 22

MONTH: Jan

YEAR: 2007

Construction of conjugative gene transfer system between E. coli and moderately thermophilic, extremely acidophilic Acidithiobacillus caldus MTH-04. Information

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LANGUAGE: eng

NlmUniqueID: 9431852

Construction of conjugative gene transfer system between E. coli and moderately thermophilic, extremely acidophilic Acidithiobacillus caldus MTH-04. Keywords Mesh Terms:

KEYWORDS: Plasmids

MESH TERMS: genetics

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Grant and Affiliation Information for Construction of conjugative gene transfer system between E. coli and moderately thermophilic, extremely acidophilic Acidithiobacillus caldus MTH-04.

AFFILIATION: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China. liuxiangmei@sdu.edu.cn

Country: Korea (South)

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MEDLINETA: J Microbiol Biotechnol

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