Construction of a deep coverage BAC library from Capsicum annuum, 'CM334'.

Construction of a deep coverage BAC library from Capsicum annuum, 'CM334'. Research Abstract Details 

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Construction of a deep coverage BAC library from Capsicum annuum, 'CM334'. Abstract Text:

E Y Yoo,S Kim,Y H Kim,C J Lee,B-D Kim,

A bacterial artificial chromosome (BAC) library consisting of 235,000 clones with an average insert size of 130 kb was constructed from Capsicum annuum, 'CM334'. Based on a pepper haploid genome size of 2,702 Mbp/C, the BAC library is estimated to contain approximately 12 genome equivalents and represents at least 99% of the pepper genome. Screening of the library with mitochondrial DNA probes (coxII, coxIII, atp6 and atp9) and chloroplast DNA probes (atpB, rbcL) indicated that contamination with cytoplasmic DNA was less than 0.5%. To estimate the possibility of isolating a specific clone, the library was screened with single or low-copy gene-specific probes and RFLP probes. Screening of high density BAC filters with RFLP markers linked to L (TMV resistance), y (fruit color), C2 (fruit color) and C (pungency) loci under high stringency conditions revealed that at least three positive BAC clones were found per each probe. This fact indicates that the library is highly reliable and represents a resource for map-based cloning, physical mapping, and characterization of upstream and downstream regulations of the chili pepper genes.

Construction of a deep coverage BAC library from Capsicum annuum, 'CM334'. Publishing Authors By Initials

EY Yoo,S Kim,YH Kim,CJ Lee,BD Kim,

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Construction of a deep coverage BAC library from Capsicum annuum, 'CM334'. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: TAG. Theoretical and applied genetics. Theoretisch

VOLUME: 107

Page Numbers: 540-3

Journal Abbreviation:

ISSN: 0040-5752

DAY: 14

MONTH: 05

YEAR: 2003

Construction of a deep coverage BAC library from Capsicum annuum, 'CM334'. Information

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LANGUAGE: eng

NlmUniqueID: 145600

Construction of a deep coverage BAC library from Capsicum annuum, 'CM334'. Keywords Mesh Terms:

KEYWORDS: Polymorphism, Restriction Fragment Lengt

MESH TERMS: genetics

Chemical & Substance for Abstract: Construction of a deep coverage BAC library from Capsicum annuum, 'CM334'. Information

Substance Name: Molecular Probes

Registry Number: 0

Grant and Affiliation Information for Construction of a deep coverage BAC library from Capsicum annuum, 'CM334'.

AFFILIATION: School of Plant Science, College of Agriculture and Life Sciences, and Center for Plant Molecular Genetics and Breeding Research, Seoul National University, 103 Seodoon-dong, Suweon 441-744, Korea.

Country: Germany

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MEDLINETA: Theor Appl Genet

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