Construction and identification of immortalized rat astrocyte cell line expressing enkephalin.

Construction and identification of immortalized rat astrocyte cell line expressing enkephalin. Research Abstract Details 

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Construction and identification of immortalized rat astrocyte cell line expressing enkephalin. Abstract Text:

Xu Ying,Tian Yu-Ke,Tian Xue-Bi,Yang Hui,

Objective:To provide a sound cell source for further ex-vivo gene therapy for chronic pain, we attempt to develop an immortalized rat astrocyte cell line that expresses enkephalin regulated by doxycycline. Methods:Retrovirus infection method was employed to develop an immortalized rat astrocyte cell line that could express enkephalin regulated by doxycycline. The hPPE gene expression level of immoralized astroyte cells (IAC)/ hPPE was detected by RT-PCR, indirect immunofluorescence staining and radioimmunoassay.Results:IAC carrying Tet-on system transfected with preproenkephalin gene could secrete enkephalin that was regulated by doxycycline in a dose-dependent manner and hPPE gene activation could be repeated in on-off-on cycles through administration or removal of doxycycline.Conclusion:An immortalized rat astrocyte cell line that secrete enkephalin under the control of doxycycline is established successfully, which provides a research basis for transgenic cell transplantation for analgesia.

Construction and identification of immortalized rat astrocyte cell line expressing enkephalin. Publishing Authors By Initials

X Ying,T Yu-Ke,T Xue-Bi,Y Hui,

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Construction and identification of immortalized rat astrocyte cell line expressing enkephalin. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Chinese journal of traumatology = Zhonghua chuang

VOLUME: 10

Page Numbers: 353-6

Journal Abbreviation: Chin. J. Traumatol.

ISSN: 1008-1275

DAY: 29

MONTH: Dec

YEAR: 2007

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LANGUAGE: eng

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AFFILIATION: Department of Anesthesiology, Children's Hospital of Chongqing University of Medical Sciences, Chongqing 400014, China.

Country: China

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MEDLINETA: Chin J Traumatol

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