[Construction and identification of a multiple myeloma-specific APE1 siRNA expression vector]

[Construction and identification of a multiple myeloma-specific APE1 siRNA expression vector] Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

[Construction and identification of a multiple myeloma-specific APE1 siRNA expression vector] Abstract Text:

Zhen-zhou Yang,Xing-hua Chen,Dong Wang,Ge Wang,De-bing Xiang,

OBJECTIVE: To construct a multiple myeloma (MM)-specific APE1siRNA expression vector, and detect the specific knock-down effect of the siRNA on expression of APE1 protein. METHODS: APE1siRNA cDNA sequence was designed, synthesized and inserted into pSilencer 2.0-U6 linear expression vector. pSilencer APE1siRNA was digested by enzyme EcoRI and BamHI, then linear vector and IgP fragments were conjugated by T4 DNA ligase. pSilencer IgP-APE1siRNA and pSilencer IE-IgP-APE1siRNA were digested by enzyme EcoRI or XhoI. Linear vector and IE or Kappa fragments were conjugated by T4 DNA ligase. Then a MM specific pSilencer K-IE-IgP-APE1siRNA was cloned. The recombinant products were identified by DNA sequencing and enzyme digestions at each step. pSilencer K-IE-IgP-APE1siRNA plasmid was transfected to KM3, HOS, MDA-231 cells by liposome. APE1 gene silence induced by RNAi was analysed by Western blot. RESULTS: APE1 protein in KM3 cells could be knocked down effectively and specifically by pSilencer K-IE-IgP-APE1siRNA vector. After 2 days, the level of APE1 protein in KM3 cells transfected with siRNA was 0.118 +/- 0.047, while that transfected with plasmid only was 0.988 +/- 0.029. The efficiency of gene silence was 90%. CONCLUSION: A MM specific APE1siRNA expression vector was successfully constructed.

[Construction and identification of a multiple myeloma-specific APE1 siRNA expression vector] Publishing Authors By Initials

ZZ Yang,XH Chen,D Wang,G Wang,DB Xiang,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

[Construction and identification of a multiple myeloma-specific APE1 siRNA expression vector] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zhonghua xue ye xue za zhi = Zhonghua xueyexue zaz

VOLUME: 27

Page Numbers: 235-9

Journal Abbreviation:

ISSN: 0253-2727

DAY: 31

MONTH: Apr

YEAR: 2006

[Construction and identification of a multiple myeloma-specific APE1 siRNA expression vector] Information

Number of References:

LANGUAGE: chi

NlmUniqueID: 8212398

[Construction and identification of a multiple myeloma-specific APE1 siRNA expression vector] Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: [Construction and identification of a multiple myeloma-specific APE1 siRNA expression vector] Information

Substance Name:

Registry Number:

Grant and Affiliation Information for [Construction and identification of a multiple myeloma-specific APE1 siRNA expression vector]

AFFILIATION: Center of Oncology, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China.

Country: China

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Zhonghua Xue Ye Xue Za Zhi

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Construction and identification of a multiple myeloma-specific APE1 siRNA expression vector Related Publications

• A comparison of nonfatal unintentional injuries in the United States among U.S.-born and foreign-born persons.
• Immune tolerance induced by adoptive transfer of dendritic cells in an insulin-dependent diabetes mellitus murine model.
• Vaccinia virus A6L encodes a virion core protein required for formation of mature virion.
• Variola virus IL-18 binding protein interacts with three human IL-18 residues that are part of a binding site for human IL-18 receptor alpha subunit.
• [A quantum chemistry investigation on antimalarial mechanism of Qinghaosu based on cleavage of the peroxide bridge]
• [Atmospheric water vapor conditions of Cnaphalocrocis medinalis outbreak in 2003]
• [Chronic renal disease in diabetic and prediabetic subjects: a community-based study in Shanghai]
• [Clinical study on the relationship among the dermis, fat dome and postburn hyperplastic scar formation]
• [Cloning and analysis of genes in the halocin C8 gene cluster]
• [Comments on the current status of the clinical application of artificial livers]
• [Comparative study on influence of enteral and parenteral nutrition on organ function in critically ill patients]
• [Construction and identification of a multiple myeloma-specific APE1 siRNA expression vector]
• [Construction of infectious cDNA clone of cucumber mosaic virus satellite RNA XJs1 and preliminary study on its biological function]
• [Effect of immunostimulatory sequence on the expression of EOTAXIN of experimental allergic rhinitis]
• [Evaluation of the four simple methods in the diagnosis of diabetic peripheral neuropathy]
• [Expression of chemokine monokine induced by interferon-gama in patients with chronic hepatitis B]
• [Facial nerve monitoring in parotid gland surgery]
• [Female urogenital mycoplasma infection and drug sensitivity status in Changsha]
• [Investigation on spectroscopic properties of Yb3+ -doped laser glasses]
• [Photo-fenton oxidation processes used in the degradation of rhodamine B]
• [Scanning HNF-1 alpha gene mutation in Chinese early-onset and/or multiplex diabetes pedigrees]
• [Scanning the HNF4A gene mutation from Chinese pedigrees with early- and/or multiple-onset diabetes]
• [Spectroscopic studies on the binding of prulifloxacin and ct-DNA]
• [Studies on the contents of twenty elements in different parts of garlic by using ICP-OES, AAS and AFS combined with microwave decomposition method]
• [Study on the characteristics and compositions of fine particles left in the solution after decomposition of plant samples]
• [Study on the determination of trace caesium in bittern by extraction separation graphite furnace atomic absorption spectrometry]
• [Study on the relationship between glutathione S transferase mu gene deletion and leukemia in workers exposed to benzene]
• [The preparation and spectroscopic characteristics of ytterbium silicate glasses]
• [The progress in research for human pulse system modeling and pulse condition information analysis]
• [Zinc deficiency inhibited the proliferation and differentiation of rat osteoblast in vitro]