Construction and characterization of monoclonal antibodies specific for the R transactivator 185 of Epstein-Barr virus.

Construction and characterization of monoclonal antibodies specific for the R transactivator 185 of Epstein-Barr virus. Research Abstract Details 

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Construction and characterization of monoclonal antibodies specific for the R transactivator 185 of Epstein-Barr virus. Abstract Text:

Yin-Hua Zhu,Ye-Sheng Wei,Hong Li,Wei-Bo Liang,Bing Du,Geng-Qian Zhang,Lin Zhang,

Epstein-Barr virus (EBV) has been implicated in the pathogenesis of several human malignancies including B lymphomas and nasopharyngeal carcinoma. The EBV R transactivator (Rta) has been found to play essential roles in stimulating a lytic cycle and viral gene expression. Recently, it was shown that ELISA detecting serum IgG-Rta(150+185) (two internal fragments of Rta) levels may be useful as a serological parameter to assist in the diagnosis of nasopharyngeal carcinoma. The present studies were to prepare monoclonal antibodies specific for the Rta185 and provide a useful tool for the detection of Rta. For this purpose, two monoclonal antibodies (Mabs) specific for the Rta185 were generated. They were identified by Western blot, enzyme-linked immunosorbent assay (ELISA) and immunofluorescence analysis. The results revealed two different immunofluorescence patterns in EBV-positive B cells and epithelial cells, and suggested that there might be a difference in EBV replication mode between B cells and epithelial cells. The Mabs obtained in this study have a potential for the diagnosis of EBV associated diseases.

Construction and characterization of monoclonal antibodies specific for the R transactivator 185 of Epstein-Barr virus. Publishing Authors By Initials

YH Zhu,YS Wei,H Li,WB Liang,B Du,GQ Zhang,L Zhang,

For similar proteins: viral proteins research abstracts see: proteins: viral proteins research

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Construction and characterization of monoclonal antibodies specific for the R transactivator 185 of Epstein-Barr virus. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of virological methods

VOLUME: 144

Page Numbers: 12-6

Journal Abbreviation: J. Virol. Methods

ISSN: 0166-0934

DAY: 29

MONTH: 05

YEAR: 2007

Construction and characterization of monoclonal antibodies specific for the R transactivator 185 of Epstein-Barr virus. Information

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LANGUAGE: eng

NlmUniqueID: 8005839

Construction and characterization of monoclonal antibodies specific for the R transactivator 185 of Epstein-Barr virus. Keywords Mesh Terms:

KEYWORDS: Viral Proteins

MESH TERMS: immunology

Chemical & Substance for Abstract: Construction and characterization of monoclonal antibodies specific for the R transactivator 185 of Epstein-Barr virus. Information

Substance Name: Viral Proteins

Registry Number: 0

Grant and Affiliation Information for Construction and characterization of monoclonal antibodies specific for the R transactivator 185 of Epstein-Barr virus.

AFFILIATION: Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan, China.

Country: Netherlands

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MEDLINETA: J Virol Methods

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