Construct synthetic gene encoding artificial spider dragline silk protein and its expression in milk of transgenic mice.

Construct synthetic gene encoding artificial spider dragline silk protein and its expression in milk of transgenic mice. Research Abstract Details 

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Construct synthetic gene encoding artificial spider dragline silk protein and its expression in milk of transgenic mice. Abstract Text:

Hong-Tao Xu,Bao-Liang Fan,Shu-Yang Yu,Yin-Hua Huang,Zhi-Hui Zhao,Zheng-Xing Lian,Yun-Ping Dai,Li-Li Wang,Zhao-Liang Liu,Jing Fei,Ning Li,Hong-Tao Xu,Bao-Liang Fan,Shu-Yang Yu,Yin-Hua Huang,Zhi-Hui Zhao,Zheng-Xing Lian,Yun-Ping Dai,Li-Li Wang,Zhao-Liang Liu,Jing Fei,Ning Li,

Based on the known partial cDNA sequence of dragline silk protein an artificial gene monomer, a 360 bp sequence, was designed and polymerized to encode an analog of dragline silk protein. Six tandem copies of monomer were cloned into pBC1 vector and microinjected into the pronuclei of fertilized Kunming White eggs. Transgenic mice were screened by Polymerase Chain Reaction (PCR) and Southern blot which revealed that 10 mice (5 male, 5 female) among 58 mice were transgenic positive. Milk of five F0 mice and eight F1 mice was analyzed by Western blot, and two F0 mice and seven F1 mice expressed recombinant dragline silk protein. In transgenic mice milk a maximum of concentration of recombinant dragline silk protein was 11.7 mg/L by radioimmunoassay.

Construct synthetic gene encoding artificial spider dragline silk protein and its expression in milk of transgenic mice. Publishing Authors By Initials

HT Xu,BL Fan,SY Yu,YH Huang,ZH Zhao,ZX Lian,YP Dai,LL Wang,ZL Liu,J Fei,N Li,HT Xu,BL Fan,SY Yu,YH Huang,ZH Zhao,ZX Lian,YP Dai,LL Wang,ZL Liu,J Fei,N Li,

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Construct synthetic gene encoding artificial spider dragline silk protein and its expression in milk of transgenic mice. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Animal biotechnology

VOLUME: 18

Page Numbers: 1-12

Journal Abbreviation: Anim. Biotechnol.

ISSN: 1532-2378

DAY: 16

MONTH: 03

YEAR: 2007

Construct synthetic gene encoding artificial spider dragline silk protein and its expression in milk of transgenic mice. Information

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LANGUAGE: eng

NlmUniqueID: 9011409

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Grant and Affiliation Information for Construct synthetic gene encoding artificial spider dragline silk protein and its expression in milk of transgenic mice.

AFFILIATION: State Key Laboratory for Agrobiotechnology, China Agriculture University, Beijing, PR China.

Country: United States

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MEDLINETA: Anim Biotechnol

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