Constitutive overexpression of Nrf2-dependent heme oxygenase-1 in A549 cells contributes to resistance to apoptosis induced by epigallocatechin 3-gallate.

Constitutive overexpression of Nrf2-dependent heme oxygenase-1 in A549 cells contributes to resistance to apoptosis induced by epigallocatechin 3-gallate. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Constitutive overexpression of Nrf2-dependent heme oxygenase-1 in A549 cells contributes to resistance to apoptosis induced by epigallocatechin 3-gallate. Abstract Text:

Mee-Hyang Kweon,Vaqar Mustafa Adhami,Jeong-Sang Lee,Hasan Mukhtar,

Epigallocatechin 3-gallate (EGCG), the major polyphenol found in green tea, exerts antiproliferative and proapoptotic effects in many cancer cells. However, we found that among many cancer cells human lung adenocarcinoma A549 cells are markedly resistant to apoptosis induction by EGCG (even at 100 microm for 72 h). Heme oxygenase-1 (HO-1) induced by stress stimuli represents a prime cellular defense mechanism, but it may be associated with enhanced cell proliferation and chemoresistance in some cancer cells. Because we found that A549 cells constitutively overexpress HO-1 and its associated transcription factor Nrf2, we tested an hypothesis that EGCG resistance in these cells may be linked with Nrf2-mediated HO-1 overexpression. HO-1 inhibition with tin-protoporphyrin IX and silencing with RNA interference rendered cells more sensitive to apoptosis induction by EGCG and classical prooxidants. Interestingly, EGCG at high concentration (>200 microm) induced apoptosis by suppressing expression of HO-1 protein and mRNA, and this effect correlated with a decrease in both Nrf2-ARE binding and HO-1-ARE-luciferase activity, suggesting Nrf2-driven transcriptional activation of ho-1. Because we observed notably high levels of phosphorylated protein kinase C alpha and its suppression by EGCG and deferoxamine (an iron chelator), a possible mechanism involving phosphorylated protein kinase C alpha and iron in Nrf2-HO-1 activation was further investigated. Collectively, our findings suggest that Nrf2-mediated HO-1 overexpression confers resistance to apoptosis induction by EGCG; therefore, its inactivation may be a target for overcoming the resistance to chemoprevention and chemotherapy.

Constitutive overexpression of Nrf2-dependent heme oxygenase-1 in A549 cells contributes to resistance to apoptosis induced by epigallocatechin 3-gallate. Publishing Authors By Initials

MH Kweon,VM Adhami,JS Lee,H Mukhtar,

For similar genetic processes: gene expression regulation: trans-activation (genetics) research abstracts see: genetic processes: gene expression regulation: trans-activation (genetics) research

PUBMED ID PMID:

MEDLINE DATE:

Constitutive overexpression of Nrf2-dependent heme oxygenase-1 in A549 cells contributes to resistance to apoptosis induced by epigallocatechin 3-gallate. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of biological chemistry

VOLUME: 281

Page Numbers: 33761-72

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 1

MONTH: 09

YEAR: 2006

Constitutive overexpression of Nrf2-dependent heme oxygenase-1 in A549 cells contributes to resistance to apoptosis induced by epigallocatechin 3-gallate. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Constitutive overexpression of Nrf2-dependent heme oxygenase-1 in A549 cells contributes to resistance to apoptosis induced by epigallocatechin 3-gallate. Keywords Mesh Terms:

KEYWORDS: Trans-Activation (Genetics)

MESH TERMS: genetics

Chemical & Substance for Abstract: Constitutive overexpression of Nrf2-dependent heme oxygenase-1 in A549 cells contributes to resistance to apoptosis induced by epigallocatechin 3-gallate. Information

Substance Name: Protein Kinase C-alpha

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Constitutive overexpression of Nrf2-dependent heme oxygenase-1 in A549 cells contributes to resistance to apoptosis induced by epigallocatechin 3-gallate.

AFFILIATION: Department of Dermatology, University of Wisconsin, Madison, Wisconsin 53706, USA.

Country: United States

AGENCY: United States NCI

GRANT: R01-CA78809

ACRONYM: CA

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Constitutive overexpression of Nrf2-dependent heme oxygenase-1 in A549 cells contributes to resistance to apoptosis induced by epigallocatechin 3-gallate Related Publications

• A novel antioxidant 3-O-Caffeoyl-1-methylquinic acid enhances ultraviolet A-mediated apoptosis in immortalized HaCaT keratinocytes via Sp1-dependent transcriptional activation of p21(WAF1/Cip1).
• Apoptosis by dietary factors: the suicide solution for delaying cancer growth.
• Cancer chemoprevention through dietary antioxidants: progress and promise.
• Cannabinoids for cancer treatment: progress and promise.
• Chemoprevention of Prostate Cancer through Dietary Agents: Progress and Promise.
• Cholesterol-depleting statin drugs protect postmitotically differentiated human neurons against ethanol- and human immunodeficiency virus type 1-induced oxidative stress in vitro.
• Complete genome analysis of a highly pathogenic H5N1 influenza A virus isolated from a tiger in China.
• Constitutive overexpression of Nrf2-dependent heme oxygenase-1 in A549 cells contributes to resistance to apoptosis induced by epigallocatechin 3-gallate.
• Demographic and clinical features of diabetes mellitus in 1095 Yemeni patients.
• Dietary agents for chemoprevention of prostate cancer.
• Effects of highly active antiretroviral therapy on HIV-1-associated oral complications.
• Fisetin, a novel dietary flavonoid, causes apoptosis and cell cycle arrest in human prostate cancer LNCaP cells.
• Green tea and skin.
• Green tea polyphenol EGCG sensitizes human prostate carcinoma LNCaP cells to TRAIL-mediated apoptosis and synergistically inhibits biomarkers associated with angiogenesis and metastasis.
• Identification of Leishmania donovani as a cause of cutaneous leishmaniasis in Sudan.
• Inhibition of UVB-mediated oxidative stress and markers of photoaging in immortalized HaCaT keratinocytes by pomegranate polyphenol extract POMx.
• Inhibition of epidermal growth factor receptor signaling pathway by delphinidin, an anthocyanidin in pigmented fruits and vegetables.
• Letter: Neonatal hyperthyroidism and long-acting thyroid stimulator protector.
• Lupeol Inhibits Growth of Highly Aggressive Human Metastatic Melanoma Cells In vitro and In vivo by Inducing Apoptosis.
• MP 48 expression in red blood cells of renal transplant recipients.
• MP84 expression in the urine specimens of acute rejection renal transplant recipients.
• Neuroscience of HIV Infection - Eighth International Meeting. Basic Research and Clinical Frontiers.
• Oral consumption of pomegranate fruit extract inhibits growth and progression of primary lung tumors in mice.
• Pomegranate derived products for cancer chemoprevention.
• Pomegranate fruit extract inhibits prosurvival pathways in human A549 lung carcinoma cells and tumor growth in athymic nude mice.
• Prescribing for asthmatic children in primary care. Are we following guidelines?
• Prevention of prostate cancer through custom tailoring of chemopreventive regimen.
• Src kinase potentiates androgen receptor transactivation function and invasion of androgen-independent prostate cancer C4-2 cells.
• Tea beverage in chemoprevention and chemotherapy of prostate cancer.
• Unusual clinical presentation of a severely protruding premaxilla in patient with bilateral clefts.