Constitutive expression of cytochrome p450 genes in newly established rat hepatic cell lines.

Constitutive expression of cytochrome p450 genes in newly established rat hepatic cell lines. Research Abstract Details 

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Constitutive expression of cytochrome p450 genes in newly established rat hepatic cell lines. Abstract Text:

Masakuni Degawa,Masayuki Namiki,Naoki Yoshimoto,Masanobu Makino,Miho Iwamoto,Kiyomitsu Nemoto,Yoshiyuki Hashimoto,

Two cultured cell lines, called Kan-R1 and Kan-R2, were established from rat hepatic cells by in vitro culture with a hepatocarcinogen, 3-methoxy-4-aminoazobenzene, and examined for the gene expression of cytochrome p450 (p450) isoforms, CYP1A1, CYP1A2, CYP2B1, CYP2B2, CYP3A1 and CYP3A2, by the RT-PCR method. It was revealed that all the p450 genes examined were expressed in both cell lines, although the two cell lines differed in cell size and colony-forming ability on a soft agar. The expression levels of the CYP1A2, CYP2B1, CYP2B2, CYP3A1, and CYP3A2 genes were lower than those in liver tissues, while that of CYP1A1 was higher in the cell lines. In both cell lines, cycloheximide, an inhibitor of protein synthesis, augmented the gene expression of the p450s except CYP2B1. These findings indicate that the newly established hepatic cell lines substantially express the p450 genes for CYP1A1, CYP1A2, CYP2B1, CYP2B2, CYP3A1, and CYP3A2, and that the constitutive gene expression of these p450s, with the exception of CYP2B1, may be inhibited by negative transcription factors.

Constitutive expression of cytochrome p450 genes in newly established rat hepatic cell lines. Publishing Authors By Initials

M Degawa,M Namiki,N Yoshimoto,M Makino,M Iwamoto,K Nemoto,Y Hashimoto,

For similar organic chemicals: azo compounds: p-aminoazobenzene research abstracts see: organic chemicals: azo compounds: p-aminoazobenzene research

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Constitutive expression of cytochrome p450 genes in newly established rat hepatic cell lines. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 133

Page Numbers: 825-31

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jun

YEAR: 2003

Constitutive expression of cytochrome p450 genes in newly established rat hepatic cell lines. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Constitutive expression of cytochrome p450 genes in newly established rat hepatic cell lines. Keywords Mesh Terms:

KEYWORDS: p-Aminoazobenzene

MESH TERMS: chemistry

Chemical & Substance for Abstract: Constitutive expression of cytochrome p450 genes in newly established rat hepatic cell lines. Information

Substance Name: Cytochrome P-450 Enzyme System

Registry Number: 9035-51-2

Grant and Affiliation Information for Constitutive expression of cytochrome p450 genes in newly established rat hepatic cell lines.

AFFILIATION: Department of Molecular Toxicology and COE Program in the 21st Century, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Shizuoka 422-8526. degawa@smail.u-shizuoka-ken.ac.jp

Country: Japan

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MEDLINETA: J Biochem

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