Constitutive Coexpression of Bacillus Endoxylanase and Trichoderma Endoglucanase Genes in Saccharomyces cerevisiae.

Constitutive Coexpression of Bacillus Endoxylanase and Trichoderma Endoglucanase Genes in Saccharomyces cerevisiae. Research Abstract Details 

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Constitutive Coexpression of Bacillus Endoxylanase and Trichoderma Endoglucanase Genes in Saccharomyces cerevisiae. Abstract Text:

Jae Hyung Lee,Myung-Ye Lim,Mi-Jin Kim,Sun-Yeon Heo,Jin-Ho Seo,Yeon-Hee Kim,Soo-Wan Nam,Jae Hyung Lee,Myung-Ye Lim,Mi-Jin Kim,Sun-Yeon Heo,Jin-Ho Seo,Yeon-Hee Kim,Soo-Wan Nam,Jae Hyung Lee,Myung-Ye Lim,Mi-Jin Kim,Sun-Yeon Heo,Jin-Ho Seo,Yeon-Hee Kim,Soo-Wan Nam,

The endoxylanase (GenBank Access No. U51675) of Bacillus spp. and endoglucanase (GenBank Access No. AY466436) of Trichoderma spp. were separately inserted downstream of the yeast constitutive ADH1 promoter, resulting in three different plasmids (pAGX1, pAGX2, and pAGX3) according to the transcription direction of two genes. When the yeast transformants, S. cerevisiae SEY2102 harboring each expression plasmid, were grown on YPD medium, the total activities of the enzymes were approximately 3.01 unit/ml, 3.24 unit/ml, and 7.56 unit/ml for endoxylanase and 0.60 unit/ml, 0.54 unit/ml, and 0.39 unit/ ml for endoglucanase, in the following order: the pAGX1, pAGX2, and pAGX3. More than 70% of the endoxylanase and endoglucanase activities was found in the extracellular media.

Constitutive Coexpression of Bacillus Endoxylanase and Trichoderma Endoglucanase Genes in Saccharomyces cerevisiae. Publishing Authors By Initials

JH Lee,MY Lim,MJ Kim,SY Heo,JH Seo,YH Kim,SW Nam,JH Lee,MY Lim,MJ Kim,SY Heo,JH Seo,YH Kim,SW Nam,JH Lee,MY Lim,MJ Kim,SY Heo,JH Seo,YH Kim,SW Nam,

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Constitutive Coexpression of Bacillus Endoxylanase and Trichoderma Endoglucanase Genes in Saccharomyces cerevisiae. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of microbiology and biotechnology

VOLUME: 17

Page Numbers: 2076-80

Journal Abbreviation: J. Microbiol. Biotechnol.

ISSN: 1017-7825

DAY: 2

MONTH: Dec

YEAR: 2007

Constitutive Coexpression of Bacillus Endoxylanase and Trichoderma Endoglucanase Genes in Saccharomyces cerevisiae. Information

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LANGUAGE: eng

NlmUniqueID: 9431852

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AFFILIATION: Department of Biomaterial Control (BK21 Program), Dong-Eui University, Busan 614-714, Korea.

Country: Korea (South)

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MEDLINETA: J Microbiol Biotechnol

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