Constitution and quantity of lysis buffer alters outcome of reverse phase protein microarrays.

Constitution and quantity of lysis buffer alters outcome of reverse phase protein microarrays. Research Abstract Details 

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Constitution and quantity of lysis buffer alters outcome of reverse phase protein microarrays. Abstract Text:

Mary Winters,Bhavana Dabir,Minshu Yu,Elise C Kohn,Mary Winters,Bhavana Dabir,Minshu Yu,Elise C Kohn,Mary Winters,Bhavana Dabir,Minshu Yu,Elise C Kohn,

Application of novel technology to clinical samples requires optimization of procedures. Reverse phase protein lysate arrays use femtomolar quantities of tissue lysate from clinical samples with which to profile biochemical events happening in the tumor. We analyzed the effects of different tissue solubilization buffers on frozen ovarian tumor samples in order to identify the system with the best signal intensity dynamic range, reproducibility, tissue solubility, and signal consistency. A modified RIPA-like buffer supplemented with DTT and SDS was deemed optimal.

Constitution and quantity of lysis buffer alters outcome of reverse phase protein microarrays. Publishing Authors By Initials

M Winters,B Dabir,M Yu,EC Kohn,M Winters,B Dabir,M Yu,EC Kohn,M Winters,B Dabir,M Yu,EC Kohn,

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Constitution and quantity of lysis buffer alters outcome of reverse phase protein microarrays. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Proteomics

VOLUME: 7

Page Numbers: 4066-8

Journal Abbreviation: Proteomics

ISSN: 1615-9853

DAY: 20

MONTH: Nov

YEAR: 2007

Constitution and quantity of lysis buffer alters outcome of reverse phase protein microarrays. Information

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LANGUAGE: eng

NlmUniqueID: 101092707

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AFFILIATION: Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA.

Country: Germany

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MEDLINETA: Proteomics

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