Conserved structure, regulatory elements, and transcriptional regulation from the GATA-1 gene testis promoter.

Conserved structure, regulatory elements, and transcriptional regulation from the GATA-1 gene testis promoter. Research Abstract Details 

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Conserved structure, regulatory elements, and transcriptional regulation from the GATA-1 gene testis promoter. Abstract Text:

K Onodera,K Yomogida,N Suwabe,S Takahashi,Y Muraosa,N Hayashi,E Ito,L Gu,M Rassoulzadegan,J D Engel,M Yamamoto,

Transcription factor GATA-1 was first identified in erythroid cells, but was later shown to also be expressed in Sertoli cells of the mouse testis. GATA-1 transcription in testis initiates from a different first exon (exon IT) than the erythroid mRNA (transcribed from exon IE). To begin to address the question of how expression of GATA-1 might be differentially regulated in Sertoli and erythroid cells, we have cloned and determined the structure of the IT promoters of both the rat and mouse GATA-1 genes. The transcription regulatory mechanism(s) controlling the synthesis of exon IT-derived mRNA was investigated by transfection of wild-type and mutant reporter genes, with and without co-transfected GATA factor expression plasmids, into either fibroblasts or Sertoli cell lines. Two GATA binding sites in the IT promoter were found to be required for GATA factor-mediated activation in fibroblasts: GATA-IT-directed reporter gene expression was activated only after co-transfection with GATA-1, implying that transcriptional activation of GATA-1 in the testis might be at least partially mediated through these GATA regulatory elements. We also found that the endogenous GATA-1 gene was silent in primary culture and two different Sertoli cell lines, and that the repression of co-transfected GATA-1 reporter genes could not be relieved by forced expression of GATA-1 in Sertoli cells. Thus the GATA-IT promoter may be under the control of a regulatory network in Sertoli cells which involves both positive and negative regulation of transcription, and conserved GATA motifs found in the IT promoter may be required for transducing these effects.

Conserved structure, regulatory elements, and transcriptional regulation from the GATA-1 gene testis promoter. Publishing Authors By Initials

K Onodera,K Yomogida,N Suwabe,S Takahashi,Y Muraosa,N Hayashi,E Ito,L Gu,M Rassoulzadegan,JD Engel,M Yamamoto,

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Conserved structure, regulatory elements, and transcriptional regulation from the GATA-1 gene testis promoter. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of biochemistry

VOLUME: 121

Page Numbers: 251-63

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1997

Conserved structure, regulatory elements, and transcriptional regulation from the GATA-1 gene testis promoter. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Conserved structure, regulatory elements, and transcriptional regulation from the GATA-1 gene testis promoter. Keywords Mesh Terms:

KEYWORDS: Zinc Fingers

MESH TERMS: genetics

Chemical & Substance for Abstract: Conserved structure, regulatory elements, and transcriptional regulation from the GATA-1 gene testis promoter. Information

Substance Name: Luciferases

Registry Number: EC 1.13.12.-

Grant and Affiliation Information for Conserved structure, regulatory elements, and transcriptional regulation from the GATA-1 gene testis promoter.

AFFILIATION: Department of Biochemistry, Tohoku University, School of Medicine, Sendai.

Country: JAPAN

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MEDLINETA: J Biochem

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