Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer.

Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer. Abstract Text:

Human Nedd8-activating enzyme AppBp1-Uba3 was purified to apparent homogeneity from erythrocytes. In the presence of [2,8-3H]ATP and 125I-Nedd8, heterodimer rapidly forms a stable stoichiometric ternary complex composed of tightly bound Nedd8 [3H]adenylate and Uba3-125I-Nedd8 thiol ester. Isotope exchange kinetics show that the heterodimer follows a pseudo-ordered mechanism with ATP the leading and Nedd8 the trailing substrate. Human AppBp1-Uba3 follows hyperbolic kinetics for HsUbc12 transthiolation with 125I-Nedd8 (kcat = 3.5 +/- 0.2 s-1), yielding Km values for ATP (103 +/- 12 microm), 125I-Nedd8 (0.95 +/- 0.18 microm), and HsUbc12 (43 +/- 13 nm) similar to those for ubiquitin activation by Uba1. Wild type 125I-ubiquitin fails to support AppBp1-Uba3 catalyzed activation or HsUbc12 transthiolation. However, modest inhibition of 125I-Nedd8 ternary complex formation by unlabeled ubiquitin suggests a Kd > 300 microm for ubiquitin. Alanine 72 of Nedd8 is a critical specificity determinant for AppBp1-Uba3 binding because 125I-UbR72L undergoes heterodimer-catalyzed hyperbolic HsUbc12 transthiolation and yields Km = 20 +/- 9 microm and kcat = 0.9 +/- 0.3 s-1. These observations demonstrate remarkable conservation in the mechanism of AppBp1-Uba3 that mirrors its sequence conservation with the Uba1 ubiquitin-activating enzyme.

Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer. Publishing Authors By Initials

For similar proteins: ubiquitins research abstracts see: proteins: ubiquitins research

PUBMED ID PMID:

MEDLINE DATE:

Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: The Journal of biological chemistry

VOLUME: 278

Page Numbers: 26823-30

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 10

MONTH: 05

YEAR: 2003

Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer. Keywords Mesh Terms:

KEYWORDS: Ubiquitins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer. Information

Substance Name: Ubiquitin-Activating Enzymes

Registry Number: EC 6.3.2.19

Grant and Affiliation Information for Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer.

AFFILIATION: Department of Biochemistry, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM34009

ACRONYM: GM

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer Related Publications

• A Rebuttal to Chiropractic Radiologists' View of the 50-year-old, Linear-No-Threshold Radiation Risk Model.
• Abnormalities on the neurological examination and EEG in young children with pervasive developmental disorders.
• Angiotensin receptor blockers on the formularies of Medicare drug plans.
• Association of breastfeeding with maternal depressive symptoms.
• COMPARATIVE SUITABILITY OF THE FOUR MURINE RODENTS OF HAWAII AS HOSTS FOR XENOPSYLLA VEXABILIS AND X. CHEOPIS (SIPHONAPTERA).
• Effect of concomitantly administered rifampin on the pharmacokinetics and safety of atazanavir administered twice daily.
• EstB-mediated hydrolysis of the siderophore triacetylfusarinine C optimizes iron uptake of Aspergillus fumigatus.
• Evidence-based protocol for structural rehabilitation of the spine and posture: review of clinical biomechanics of posture (CBP) publications.
• H pylori infection causes chronic pancreatitis in Mongolian gerbils.
• HIF-1alpha and HIF-2alpha play a central role in stretch-induced but not shear-stress-induced angiogenesis in rat skeletal muscle.
• Interaction of HapX with the CCAAT-binding complex--a novel mechanism of gene regulation by iron.
• JCCA 2006;50 (3):172-181. Oakley P, Harrison DD, Harrison DE, Haas J. A Rebuttal to Chiropractic Radiologists' View of the 50 year old, Linear-No-Threshold Radiation Risk Model (Commentary).
• Long-term depression of cortico-striatal synaptic transmission by DHPG depends on endocannabinoid release and nitric oxide synthesis.
• Low-turnover bone disease in hypercalcemic hyperparathyroidism after kidney transplantation.
• Maternal shoe size and infant birth weight: correlation or fiction?
• Measurement of infection control department performance: state of the science.
• Measuring managed care and its environment using national surveys: a review and assessment.
• Mechanism of hcnA mRNA recognition in the Gac/Rsm signal transduction pathway of Pseudomonas fluorescens.
• Mott glass in site-diluted S=1 antiferromagnets with single-ion anisotropy.
• Myxozoan transmission via actinospores: new insights into mechanisms and adaptations for host invasion.
• On "phantom risks" associated with diagnostic ionizing radiation: evidence in support of revising radiography standards and regulations in chiropractic.
• Predictive value of sputum gram stain for the determination of appropriate antibiotic therapy in ventilator-associated pneumonia.
• Quantum glass phases in the disordered Bose-Hubbard model.
• Race/ethnicity, socioeconomic status and the health of pregnant women.
• Rates and predictors of colorectal cancer screening.
• Standard surgical versus percutaneous dilatational tracheostomy in intensive care patients.
• The long-term effects of poor childhood health: an assessment and application of retrospective reports.
• Use of Fallacious Arguments, Ad Hominem Attacks, and Biased 'Expert Opinions' Can Make CBP Research 'Appear Flawed'.
• Voriconazole: a broad spectrum triazole for the treatment of serious and invasive fungal infections.
• X-ray diffraction from paclitaxel-loaded zwitterionic and cationic model membranes.