Conservation and diversity among the three-dimensional folds of the Dicistroviridae intergenic region IRESes.

Conservation and diversity among the three-dimensional folds of the Dicistroviridae intergenic region IRESes. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Conservation and diversity among the three-dimensional folds of the Dicistroviridae intergenic region IRESes. Abstract Text:

Jennifer S Pfingsten,David A Costantino,Jeffrey S Kieft,

Internal ribosome entry site (IRES) RNAs are necessary for successful infection of many pathogenic viruses, but the details of the RNA structure-based mechanism used to bind and manipulate the ribosome remain poorly understood. The IRES RNAs from the Dicistroviridae intergenic region (IGR) are an excellent model system to understand the fundamental tenets of IRES function, requiring no protein factors to manipulate the ribosome and initiate translation. Here, we explore the architecture of four members of the IGR IRESes, representative of the two divergent classes of these IRES RNAs. Using biochemical and structural probing methods, we show that despite sequence variability they contain a common three-dimensional fold. The three-dimensional architecture of the ribosome binding domain from these IRESes is organized around a core helical scaffold, around which the rest of the RNA molecule folds. However, subtle variation in the folds of these IRESes and the presence of an additional secondary structure element suggest differences in the details of their manipulation of the large ribosomal subunit. Overall, the results demonstrate how a conserved three-dimensional RNA fold governs ribosome binding and manipulation.

Conservation and diversity among the three-dimensional folds of the Dicistroviridae intergenic region IRESes. Publishing Authors By Initials

JS Pfingsten,DA Costantino,JS Kieft,

For similar cells: cellular structures: intracellular space: cytoplasm: cytoplasmic structures: organelles: ribosomes research abstracts see: cells: cellular structures: intracellular space: cytoplasm: cytoplasmic structures: organelles: ribosomes research

PUBMED ID PMID:

MEDLINE DATE:

Conservation and diversity among the three-dimensional folds of the Dicistroviridae intergenic region IRESes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of molecular biology

VOLUME: 370

Page Numbers: 856-69

Journal Abbreviation: J. Mol. Biol.

ISSN: 0022-2836

DAY: 8

MONTH: 05

YEAR: 2007

Conservation and diversity among the three-dimensional folds of the Dicistroviridae intergenic region IRESes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985088

Conservation and diversity among the three-dimensional folds of the Dicistroviridae intergenic region IRESes. Keywords Mesh Terms:

KEYWORDS: Ribosomes

MESH TERMS: genetics

Chemical & Substance for Abstract: Conservation and diversity among the three-dimensional folds of the Dicistroviridae intergenic region IRESes. Information

Substance Name: RNA, Viral

Registry Number: 0

Grant and Affiliation Information for Conservation and diversity among the three-dimensional folds of the Dicistroviridae intergenic region IRESes.

AFFILIATION: Department of Biochemistry and Molecular Genetics, University of Colorado at Denver and Health Sciences Center, Mail stop 8101, PO Box 6511, Aurora, CO 80045, USA.

Country: England

AGENCY: United States NIGMS

GRANT: R01 GM072560-03

ACRONYM: GM

MEDLINETA: J Mol Biol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Conservation and diversity among the three-dimensional folds of the Dicistroviridae intergenic region IRESes Related Publications

• A general strategy to solve the phase problem in RNA crystallography.
• Conservation and diversity among the three-dimensional folds of the Dicistroviridae intergenic region IRESes.
• Improved native affinity purification of RNA.
• Micturition syncope.
• RNA structure-based ribosome recruitment: lessons from the Dicistroviridae intergenic region IRESes.
• Viral IRES RNA structures and ribosome interactions.
• [Spinal epidural abscess as a complication of a finger infection]
• tRNA-mRNA mimicry drives translation initiation from a viral IRES.