Consecutive analysis of sphingoglycolipids on the basis of sugar and ceramide moieties by high performance liquid chromatography.

Consecutive analysis of sphingoglycolipids on the basis of sugar and ceramide moieties by high performance liquid chromatography. Research Abstract Details 

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Consecutive analysis of sphingoglycolipids on the basis of sugar and ceramide moieties by high performance liquid chromatography. Abstract Text:

T Yamazaki,A Suzuki,S Handa,T Yamakawa,

A quantitative consecutive method was developed for analysis of sphingoglycolipids in biological materials by high performance liquid chromatography (HPLC). Crude lipid extracts were separated into neutral and acidic fractions on a DEAE-Sephadex column. Glycolipid fractions were obtained by acetylation and Florisil column chromatography, and the acetylated glycolipids were N-p-nitrobenzoylated by treatment with p-nitrobenzoyl chloride in pyridine at 60 degrees C for 6 h. Excess reagent and by-products were removed by solvent partition and gel filtration. The glycolipid derivatives were analyzed by their absorption at 254 nm on Zorbax SIL, a silica gel column, with a gradient of 0.5--7% isopropanol in hexane-chloroform (2 : 1, v/v) at a flow rate of 0.5 ml/min. The detector response was linear with up to 60 nmol of injected glycolipids. The practical lower limit of detection was about 50 pmol. The derivatives were separated on the basis of their sugar chains. Effluents corresponding to each peak were collected and analyzed further on the basis of their lipid portion on mu-Bondapak C18, a reversed phase column. This combined procedure was applied to the analysis of erythrocyte glycolipids. Samples containing as little as 20 micrograms of glycolipids could be analyzed by this method.

Consecutive analysis of sphingoglycolipids on the basis of sugar and ceramide moieties by high performance liquid chromatography. Publishing Authors By Initials

T Yamazaki,A Suzuki,S Handa,T Yamakawa,

For similar animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats research abstracts see: animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats research

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Consecutive analysis of sphingoglycolipids on the basis of sugar and ceramide moieties by high performance liquid chromatography. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 86

Page Numbers: 803-9

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Sep

YEAR: 1979

Consecutive analysis of sphingoglycolipids on the basis of sugar and ceramide moieties by high performance liquid chromatography. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Consecutive analysis of sphingoglycolipids on the basis of sugar and ceramide moieties by high performance liquid chromatography. Keywords Mesh Terms:

KEYWORDS: Rats

MESH TERMS: blood

Chemical & Substance for Abstract: Consecutive analysis of sphingoglycolipids on the basis of sugar and ceramide moieties by high performance liquid chromatography. Information

Substance Name: Lipids

Registry Number: 0

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Country: JAPAN

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MEDLINETA: J Biochem

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