Conjugative DNA transfer in Streptomyces: SpdB2 involved in the intramycelial spreading of plasmid pSVH1 is an oligomeric integral membrane protein that binds to dsDNA.

Conjugative DNA transfer in Streptomyces: SpdB2 involved in the intramycelial spreading of plasmid pSVH1 is an oligomeric integral membrane protein that binds to dsDNA. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Conjugative DNA transfer in Streptomyces: SpdB2 involved in the intramycelial spreading of plasmid pSVH1 is an oligomeric integral membrane protein that binds to dsDNA. Abstract Text:

Yvonne Tiffert,Birke ,Jens Reuther,Wolfgang Wohlleben, Muth,

In the current model of conjugal plasmid transfer in mycelium-forming streptomycetes, plasmid transfer by the FtsK-like TraB protein is followed by the subsequent spreading of the newly transferred plasmid within the neighbouring mycelial compartments. Several plasmid-encoded Spd proteins are involved in the plasmid spreading by an unknown mechanism. spdB2 of the conjugative pSVH1 plasmid of Streptomyces venezuelae was heterologously expressed in Escherichia coli and Streptomyces lividans, with a C-terminal His-tag-encoding sequence. Induction of spdB2-His expression affected viability in both species. The integral membrane protein SpdB2-His was eluted from the membrane fraction of S. lividans with Triton X-100, and purified as a soluble protein by Ni-NTA affinity chromatography. Cross-linking experiments with glutaraldehyde showed that SpdB2-His formed oligomers. SpdB2-His had a nonspecific DNA-binding activity: while all types of dsDNA were bound, single-stranded M13-DNA was not recognized. The spd genes of the spdB3-spd79-spdB2 operon of pSVH1 were simultaneously expressed in E. coli with different affinity tags. While expression of StrepII-SpdB3 was not detected, Spd79-flag and SpdB2-His were localized in the membrane fraction of E. coli. In the absence of SpdB2, most of the Spd79-flag protein was found in the cytoplasmic fraction, indicating that SpdB2 affects localization of Spd79. Pulldown assays with StrepII-TraB protein of pSVH1 demonstrated that TraB interacted with SpdB2, suggesting that the septal DNA translocator TraB is also involved in intramycelial plasmid spreading.

Conjugative DNA transfer in Streptomyces: SpdB2 involved in the intramycelial spreading of plasmid pSVH1 is an oligomeric integral membrane protein that binds to dsDNA. Publishing Authors By Initials

Y Tiffert,B ,J Reuther,W Wohlleben,G Muth,

For similar bacteria: endospore-forming bacteria: gram-positive endospore-forming bacteria: gram-positive endospore-forming rods: streptomycetaceae: streptomyces research abstracts see: bacteria: endospore-forming bacteria: gram-positive endospore-forming bacteria: gram-positive endospore-forming rods: streptomycetaceae: streptomyces research

PUBMED ID PMID:

MEDLINE DATE:

Conjugative DNA transfer in Streptomyces: SpdB2 involved in the intramycelial spreading of plasmid pSVH1 is an oligomeric integral membrane protein that binds to dsDNA. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Microbiology (Reading, England)

VOLUME: 153

Page Numbers: 2976-83

Journal Abbreviation: Microbiology (Reading, Engl.)

ISSN: 1350-0872

DAY: 26

MONTH: Sep

YEAR: 2007

Conjugative DNA transfer in Streptomyces: SpdB2 involved in the intramycelial spreading of plasmid pSVH1 is an oligomeric integral membrane protein that binds to dsDNA. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9430468

Conjugative DNA transfer in Streptomyces: SpdB2 involved in the intramycelial spreading of plasmid pSVH1 is an oligomeric integral membrane protein that binds to dsDNA. Keywords Mesh Terms:

KEYWORDS: Streptomyces

MESH TERMS: metabolism

Chemical & Substance for Abstract: Conjugative DNA transfer in Streptomyces: SpdB2 involved in the intramycelial spreading of plasmid pSVH1 is an oligomeric integral membrane protein that binds to dsDNA. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Conjugative DNA transfer in Streptomyces: SpdB2 involved in the intramycelial spreading of plasmid pSVH1 is an oligomeric integral membrane protein that binds to dsDNA.

AFFILIATION: Mikrobiologie/Biotechnologie, Mikrobiologisches Institut, Fakultät für Biologie, Eberhard Karls Universität Tübingen, Auf der Morgenstelle 28, 72076 Tübingen, Germany.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Microbiology

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Conjugative DNA transfer in Streptomyces: SpdB2 involved in the intramycelial spreading of plasmid pSVH1 is an oligomeric integral membrane protein that binds to dsDNA Related Publications

• Brucellosis control in Oregon.
• Chronic copper poisoning in sheep.
• Conjugative DNA transfer in Streptomyces: SpdB2 involved in the intramycelial spreading of plasmid pSVH1 is an oligomeric integral membrane protein that binds to dsDNA.
• Disubstituted pyridines: the double-coupling approach.
• Effects of brief training on use of automated external defibrillators by people without medical expertise.
• LABORATORY RELATIONS: FROM THE VIEWPOINT OF THE LABORATORY INDUSTRY.
• Large-network concepts and small-network characteristics: fixed and variable factors.
• Studies on the morphology and respiration of Anaplasma marginale.
• Total haemoglobin mass and spleen contraction: a study on competitive apnea divers, non-diving athletes and untrained control subjects.
• White muscle disease (myopathy) in lambs and calves. III. Experimental production in calves from cows fed alfalfa hay.
• [Acute kidney failure in obstetrics and gynecology. Pathologic-anatomic part]
• [An impressive case of prostate cancer metastasis to the maxilla.]
• [Deficiency symptoms after gynecological operations, despite unilateral functional conservation.]
• [Effects of acute inflammation of pelvic connective tissue on the efferent urinary ducts.]
• [Ester of carbinol and succinic acid as choleretic.]
• [Function of remaining ovary after unilateral ovarian extirpation or resection.]
• [Heart defects and delivery]
• [Influence of pyelonephritis gravidarum on the fetal development]
• [Investing in the quality of human harmony]
• [On acute kidney failure within the scope of obstetrics and gynecology]
• [On the conservative treatment of postoperative ileus]
• [Ovarian function after bilateral extirpation or resection of the fallopian tubes.]
• [Ovarian function after removal of the corpus uteri.]
• [Potentialized anesthesia.]
• [Prognosis of hydronephrosis occurring after radiotherapy and radical surgery in cervical cancer.]
• [Rapid section during labor]
• [Rare complication following the Marshall-Marchetti operation]
• [Some actual problems in biophysics.]
• [Surgical technic of sterilization]
• [Symptomatology and therapy of mediastinal lipoma.]