Conjugation of methoxypolyethylene glycol to the surface of bovine red blood cells.

Conjugation of methoxypolyethylene glycol to the surface of bovine red blood cells. Research Abstract Details 

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Conjugation of methoxypolyethylene glycol to the surface of bovine red blood cells. Abstract Text:

Sharon I Gundersen,Andre F Palmer,

Methoxypolyethylene glycol (mPEG) covalently bound to the surface of human red blood cells (hRBCs) has been shown to decrease immunological recognition of hRBC surface antigens (Bradley et al., 2002). However, there is an increasing shortage of hRBC donations, thus making hRBCs scarce and expensive (Davey, 2004; Riess, 2001). The goal of this study is to similarly PEGylate the surface of bovine RBCs (bRBCs) with the aim of reducing the demand on human blood donations needed for blood transfusions. This study investigates the feasibility of modifying the surface of bRBCs with the succinimidyl ester of methoxypolyethylene glycol propionic acid (SPA-mPEG) for use as a potential blood substitute. The oxygen binding affinity of PEGylated bRBCs was moderately increased with increasing initial SPA-mPEG concentrations up to 4 mM when reacted with bRBCs at a hematocrit of 12%. Oxygen transport simulations verified that SPA-mPEG conjugated bRBCs could still transport oxygen to pancreatic islet tissues even under extreme conditions. PEGylated bRBCs reconstituted to a hematocrit of 40% exhibited viscosities on the order of approximately 3 cp, similar to hRBCs at the same hematocrit. Taken together, the results of this study demonstrate the success of PEGylating bRBCs to yield modified cells with oxygen binding, transport and flow properties similar to that of hRBCs.

Conjugation of methoxypolyethylene glycol to the surface of bovine red blood cells. Publishing Authors By Initials

SI Gundersen,AF Palmer,

For similar investigative techniques: rheology research abstracts see: investigative techniques: rheology research

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Conjugation of methoxypolyethylene glycol to the surface of bovine red blood cells. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Biotechnology and bioengineering

VOLUME: 96

Page Numbers: 1199-210

Journal Abbreviation: Biotechnol. Bioeng.

ISSN: 0006-3592

DAY: 15

MONTH: Apr

YEAR: 2007

Conjugation of methoxypolyethylene glycol to the surface of bovine red blood cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7502021

Conjugation of methoxypolyethylene glycol to the surface of bovine red blood cells. Keywords Mesh Terms:

KEYWORDS: Rheology

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Conjugation of methoxypolyethylene glycol to the surface of bovine red blood cells. Information

Substance Name: monomethoxypolyethylene glycol

Registry Number: 9004-74-4

Grant and Affiliation Information for Conjugation of methoxypolyethylene glycol to the surface of bovine red blood cells.

AFFILIATION: Department of Biomolecular and Chemical Engineering, University of Notre Dame, 182 Fitzpatrick Hall, Notre Dame, Indiana 46656, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: HL078840

ACRONYM: HL

MEDLINETA: Biotechnol Bioeng

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