Conjugates of ubiquitin cross-reactive protein distribute in a cytoskeletal pattern.

Conjugates of ubiquitin cross-reactive protein distribute in a cytoskeletal pattern. Research Abstract Details 

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Conjugates of ubiquitin cross-reactive protein distribute in a cytoskeletal pattern. Abstract Text:

Ubiquitin cross-reactive protein (UCRP), a 15-kDa interferon-induced protein, is a sequence homolog of ubiquitin that is covalently ligated to intracellular proteins in a parallel enzymatic reaction and is found at low levels within cultured cell lines and human tissues not exposed to interferon. Ubiquitin and UCRP ligation reactions apparently target distinct subsets of intracellular proteins, as judged from differences in the distributions of the respective adducts revealed on immunoblots. In this study, successive passages of the human lung carcinoma line A549 in the presence of neutralizing antibodies against alpha and beta interferons had no effect on the levels of either free or conjugated UCRP, indicating that these UCRP pools are constitutively present within uninduced cells and are thus not a consequence of autoinduction by low levels of secreted alpha/beta interferon. In an effort to identify potential targets for UCRP conjugation, the immunocytochemical distribution of UCRP was examined by using affinity-purified polyclonal antibodies against recombinant polypeptide. UCRP distributes in a punctate cytoskeletal pattern that is resistant to extraction by nonionic detergents (e.g., Triton X-100) in both uninduced and interferon-treated A549 cells. The cytoskeletal pattern colocalizes with the intermediate filament network of epithelial and mesothelial cell lines. Immunoblots of parallel Triton X-100-insoluble cell extracts suggest that the cytoskeletal association largely results from the noncovalent association of UCRP conjugates with the intermediate filaments rather than direct ligation of the polypeptide to structural components of the filaments. A significant increase in the sequestration of UCRP adducts on intermediate filaments accompanies interferon induction. These results suggest that UCRP may serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments.

Conjugates of ubiquitin cross-reactive protein distribute in a cytoskeletal pattern. Publishing Authors By Initials

For similar proteins: ubiquitins research abstracts see: proteins: ubiquitins research

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Conjugates of ubiquitin cross-reactive protein distribute in a cytoskeletal pattern. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Molecular and cellular biology

VOLUME: 14

Page Numbers: 8408-19

Journal Abbreviation: Mol. Cell. Biol.

ISSN: 0270-7306

DAY: 6

MONTH: Dec

YEAR: 1994

Conjugates of ubiquitin cross-reactive protein distribute in a cytoskeletal pattern. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8109087

Conjugates of ubiquitin cross-reactive protein distribute in a cytoskeletal pattern. Keywords Mesh Terms:

KEYWORDS: Ubiquitins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Conjugates of ubiquitin cross-reactive protein distribute in a cytoskeletal pattern. Information

Substance Name: Keratins

Registry Number: 68238-35-7

Grant and Affiliation Information for Conjugates of ubiquitin cross-reactive protein distribute in a cytoskeletal pattern.

AFFILIATION: Department of Biochemistry, Medical College of Wisconsin, Milwaukee 53226.

Country: UNITED STATES

AGENCY: United States NIGMS

GRANT: GM47426

ACRONYM: GM

MEDLINETA: Mol Cell Biol

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