Conformational Changes below the T(m): Molecular Dynamics Studies of the Thermal Pretransition of Ribonuclease A.

Conformational Changes below the T(m): Molecular Dynamics Studies of the Thermal Pretransition of Ribonuclease A. Research Abstract Details 

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Conformational Changes below the T(m): Molecular Dynamics Studies of the Thermal Pretransition of Ribonuclease A. Abstract Text:

Eric D Merkley,Brady Bernard,Valerie Daggett,Eric D Merkley,Brady Bernard,Valerie Daggett,Eric D Merkley,Brady Bernard,Valerie Daggett,

Recent work suggests that some native conformations of proteins can vary with temperature. To obtain an atomic-level description of this structural and conformational variation, we have performed all-atom, explicit-solvent molecular dynamics simulations of bovine pancreatic ribonuclease A (RNase A) up to its melting temperature (Tm approximately 337 K). RNase A has a thermal pretransition near 320 K [Stelea, S. D., Pancoska, P., Benight, A. S., and Keiderling, T. A. (2001) Protein Sci. 10, 970-978]. Our simulations identify a conformational change that coincides with this pretransition. Between 310 and 320 K, there is a small but significant decrease in the number of native contacts, beta-sheet hydrogen bonding, and deviation of backbone conformation from the starting structure, and an increase in the number of nonnative contacts. Native contacts are lost in beta-sheet regions and in alpha1, partially due to movement of alpha1 away from the beta-sheet core. At 330 and 340 K, a nonnative helical segment of residues 15-20 forms, corresponding to a helix observed in the N-terminal domain-swapped dimer [Liu, Y. S., Hart, P. J., Schulnegger, M. P., and Eisenberg, D. (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 3437-3432]. The conformations observed at the higher temperatures possess nativelike topology and overall conformation, with many native contacts, but they have a disrupted active site. We propose that these conformations may represent the native state at elevated temperature, or the N' state. These simulations show that subtle, functionally important changes in protein conformation can occur below the Tm.

Conformational Changes below the T(m): Molecular Dynamics Studies of the Thermal Pretransition of Ribonuclease A. Publishing Authors By Initials

ED Merkley,B Bernard,V Daggett,ED Merkley,B Bernard,V Daggett,ED Merkley,B Bernard,V Daggett,

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Conformational Changes below the T(m): Molecular Dynamics Studies of the Thermal Pretransition of Ribonuclease A. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biochemistry

VOLUME: 47

Page Numbers: 880-92

Journal Abbreviation: Biochemistry

ISSN: 0006-2960

DAY: 28

MONTH: 12

YEAR: 2007

Conformational Changes below the T(m): Molecular Dynamics Studies of the Thermal Pretransition of Ribonuclease A. Information

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LANGUAGE: eng

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AFFILIATION: Department of Biochemistry and Department of Bioengineering, University of Washington, Seattle, Washington 98195-5061.

Country: United States

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MEDLINETA: Biochemistry

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