Confocal fluorescence polarization microscopy in turbid media: effects of scattering-induced depolarization.

Confocal fluorescence polarization microscopy in turbid media: effects of scattering-induced depolarization. Research Abstract Details 

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Confocal fluorescence polarization microscopy in turbid media: effects of scattering-induced depolarization. Abstract Text:

Chad E Bigelow,Thomas H Foster,

We present an experimental and theoretical study of confocal fluorescence polarization microscopy in turbid media. We have performed an experimental study using a fluorophore-embedded polymer rod immersed in aqueous suspensions of 0.1 and 0.5 microm diameter polystyrene microspheres. A Monte Carlo approach to simulate confocal fluorescence polarization imaging in scattering media is also presented. It incorporates a detailed model of polarized fluorescence generation that includes sampling of elliptical polarization, excited-state molecular rotational Brownian motion, and dipole fluorescence emission. Using both approaches, we determine the effects of the number of scattering events, target depth, photon scattering statistics, objective numerical aperture, and pinhole size on confocal anisotropy imaging. From this detailed analysis and comparison of experiment with simulation, we determine that fluorescence polarization is maintained to depths at which meaningful intensity images can be acquired.

Confocal fluorescence polarization microscopy in turbid media: effects of scattering-induced depolarization. Publishing Authors By Initials

CE Bigelow,TH Foster,

For similar investigative techniques: chemistry, analytical: scattering, radiation research abstracts see: investigative techniques: chemistry, analytical: scattering, radiation research

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Confocal fluorescence polarization microscopy in turbid media: effects of scattering-induced depolarization. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of the Optical Society of America. A, Opti

VOLUME: 23

Page Numbers: 2932-43

Journal Abbreviation:

ISSN: 1084-7529

DAY: 3

MONTH: Nov

YEAR: 2006

Confocal fluorescence polarization microscopy in turbid media: effects of scattering-induced depolarization. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9800943

Confocal fluorescence polarization microscopy in turbid media: effects of scattering-induced depolarization. Keywords Mesh Terms:

KEYWORDS: Scattering, Radiation

MESH TERMS: methods

Chemical & Substance for Abstract: Confocal fluorescence polarization microscopy in turbid media: effects of scattering-induced depolarization. Information

Substance Name: Complex Mixtures

Registry Number: 0

Grant and Affiliation Information for Confocal fluorescence polarization microscopy in turbid media: effects of scattering-induced depolarization.

AFFILIATION: Institute of Optics, University of Rochester, Rochester, New York 14627, USA. bigelow@psicorp.com

Country: United States

AGENCY: United States NCI

GRANT: CA68409

ACRONYM: CA

MEDLINETA: J Opt Soc Am A Opt Image Sci V

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