Confirmation of tick bite by detection of antibody to Ixodes calreticulin salivary protein.

Confirmation of tick bite by detection of antibody to Ixodes calreticulin salivary protein. Research Abstract Details 

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Confirmation of tick bite by detection of antibody to Ixodes calreticulin salivary protein. Abstract Text:

Francisco Alarcon-Chaidez,Raymond Ryan,Stephen Wikel,Kenneth Dardick,Caroline Lawler,Ivo M Foppa,Patricio Tomas,Alexis Cushman,Ann Hsieh,Andrew Spielman,Keith R Bouchard,Filiciano Dias,Jaber Aslanzadeh,Peter J Krause,

Ticks introduce a variety of pharmacologically active molecules into their host during attachment and feeding in order to obtain a blood meal. People who are repeatedly exposed to ticks may develop an immune response to tick salivary proteins. Despite this response, people usually are unaware of having been bitten, especially if they are not repeatedly exposed to ticks. In order to develop a laboratory marker of tick exposure that would be useful in understanding the epidemiology of tick-borne infection and the immune response to tick bite, we developed an enzyme-linked immunosorbent assay (ELISA) to detect antibody to a recombinant form of calreticulin protein found in the salivary glands of Ixodes scapularis, a member of a complex of Ixodes ticks that serve as the vectors for Lyme disease, human babesiosis, and human granulocytic anaplasmosis. Using this assay, we tested sera obtained from C3H/HeN and BALB/c mice before and after experimental deer tick infestation. These mice developed antibody to Ixodes calreticulin antigen after infestation. We then used the same assay to test sera obtained from people before and after they experienced deer tick bite(s). People experiencing deer tick bite(s) developed Ixodes calreticulin-specific antibody responses that persisted for up to 17 months. This Ixodes recombinant calreticulin ELISA provides objective evidence of deer tick exposure in people.

Confirmation of tick bite by detection of antibody to Ixodes calreticulin salivary protein. Publishing Authors By Initials

F Alarcon-Chaidez,R Ryan,S Wikel,K Dardick,C Lawler,IM Foppa,P Tomas,A Cushman,A Hsieh,A Spielman,KR Bouchard,F Dias,J Aslanzadeh,PJ Krause,

For similar proteins: salivary proteins research abstracts see: proteins: salivary proteins research

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MEDLINE DATE:

Confirmation of tick bite by detection of antibody to Ixodes calreticulin salivary protein. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Clinical and vaccine immunology : CVI

VOLUME: 13

Page Numbers: 1217-22

Journal Abbreviation: Clin. Vaccine Immunol.

ISSN: 1556-6811

DAY: 23

MONTH: 08

YEAR: 2006

Confirmation of tick bite by detection of antibody to Ixodes calreticulin salivary protein. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101252125

Confirmation of tick bite by detection of antibody to Ixodes calreticulin salivary protein. Keywords Mesh Terms:

KEYWORDS: Salivary Proteins

MESH TERMS: immunology

Chemical & Substance for Abstract: Confirmation of tick bite by detection of antibody to Ixodes calreticulin salivary protein. Information

Substance Name: Salivary Proteins

Registry Number: 0

Grant and Affiliation Information for Confirmation of tick bite by detection of antibody to Ixodes calreticulin salivary protein.

AFFILIATION: Departments of Immunology, University of Connecticut School of Medicine, Farmington, Connecticut 06030, USA.

Country: United States

AGENCY: United States NIAID

GRANT: R01 AI062735

ACRONYM: AI

MEDLINETA: Clin Vaccine Immunol

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