Conditional stimulation of type V and VI adenylyl cyclases by G protein betagamma subunits.

Conditional stimulation of type V and VI adenylyl cyclases by G protein betagamma subunits. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Conditional stimulation of type V and VI adenylyl cyclases by G protein betagamma subunits. Abstract Text:

Xianlong Gao,Rachna Sadana,Carmen W Dessauer,Tarun B Patel,

In a yeast two-hybrid screen of mouse brain cDNA library, using the N-terminal region of human type V adenylyl cyclase (hACV) as bait, we identified G protein beta2 subunit as an interacting partner. Additional yeast two-hybrid assays showed that the Gbeta(1) subunit also interacts with the N-terminal segments of hACV and human type VI adenylyl cyclase (hACVI). In vitro adenylyl cyclase (AC) activity assays using membranes of Sf9 cells expressing hACV or hACVI showed that Gbetagamma subunits enhance the activity of these enzymes provided either Galpha(s) or forskolin is present. Deletion of residues 77-151, but not 1-76, in the N-terminal region of hACVI obliterated the ability of Gbetagamma subunits to conditionally stimulate the enzyme. Likewise, activities of the recombinant, engineered, soluble forms of ACV and ACVI, which lack the N termini, were not enhanced by Gbetagamma subunits. Transfection of the C terminus of G protein receptor kinase 2 to sequester endogenous Gbetagamma subunits attenuated the ability of isoproterenol to increase cAMP accumulation in COS-7 cells overexpressing hACVI even when G(i) was inactivated by pertussis toxin. Therefore, we conclude that the N termini of human hACV and hACVI are necessary for interactions with, and regulation by, Gbetagamma subunits both in vitro and in intact cells. Moreover, Gbetagamma subunits derived from a source(s) other than G(i) are necessary for the full activation of hACVI by isoproterenol in intact cells.

Conditional stimulation of type V and VI adenylyl cyclases by G protein betagamma subunits. Publishing Authors By Initials

X Gao,R Sadana,CW Dessauer,TB Patel,

For similar investigative techniques: genetic techniques: cloning, molecular: two-hybrid system techniques research abstracts see: investigative techniques: genetic techniques: cloning, molecular: two-hybrid system techniques research

PUBMED ID PMID:

MEDLINE DATE:

Conditional stimulation of type V and VI adenylyl cyclases by G protein betagamma subunits. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 294-302

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 16

MONTH: 11

YEAR: 2006

Conditional stimulation of type V and VI adenylyl cyclases by G protein betagamma subunits. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Conditional stimulation of type V and VI adenylyl cyclases by G protein betagamma subunits. Keywords Mesh Terms:

KEYWORDS: Two-Hybrid System Techniques

MESH TERMS: chemistry

Chemical & Substance for Abstract: Conditional stimulation of type V and VI adenylyl cyclases by G protein betagamma subunits. Information

Substance Name: Adenylate Cyclase

Registry Number: EC 4.6.1.1

Grant and Affiliation Information for Conditional stimulation of type V and VI adenylyl cyclases by G protein betagamma subunits.

AFFILIATION: Department of Pharmacology and Experimental Therapeutics, Loyola University Chicago, Stritch School of Medicine, Maywood, Illinois 60153, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: HL59679

ACRONYM: HL

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Conditional stimulation of type V and VI adenylyl cyclases by G protein betagamma subunits Related Publications

• A framework for protein structure classification and identification of novel protein structures.
• A new locus for autosomal dominant amelogenesis imperfecta on chromosome 8q24.3.
• A pharmacophore derived phenytoin analogue with increased affinity for slow inactivated sodium channels exhibits a desired anticonvulsant profile.
• A potential protein-RNA recognition event along the RISC-loading pathway from the structure of A. aeolicus Argonaute with externally bound siRNA.
• A simple method for DNA isolation from clotted blood extricated rapidly from serum separator tubes.
• Acridone Alkaloids and Other Constituents of the Leaves of Atalantia ceylanica.
• Anti-inflammatory effects of isoflavones are dependent on flow and human endothelial cell PPARgamma.
• Computer-assisted scoring of the elevated plus maze.
• Conditional stimulation of type V and VI adenylyl cyclases by G protein betagamma subunits.
• Electrical stimulation to restore vestibular function development of a 3-d vestibular prosthesis.
• Epidermal growth factor receptor pathway targeted therapy in patients with aerodigestive malignancies.
• Expression of PACT is regulated by Sp1 transcription factor.
• Hydroxyamide analogs of propofol exhibit state-dependent block of sodium channels in hippocampal neurons: implications for anticonvulsant activity.
• ICAM-1 cross-linking stimulates endothelial glutathione synthesis.
• Immunohistochemical localization of dopamine receptor subtypes (D1R-D5R) in Alzheimer's disease brain.
• Interaction between glutamate and GABA systems in the integration of sympathetic outflow by the paraventricular nucleus of the hypothalamus.
• Interleukin-6 contributes to growth in cholangiocarcinoma cells by aberrant promoter methylation and gene expression.
• Pifithrin-alpha enhances chemosensitivity by a p38 mitogen-activated protein kinase-dependent modulation of the eukaryotic initiation factor 4E in malignant cholangiocytes.
• SAGA: a subgraph matching tool for biological graphs.
• Secondary mania of vascular origin in elderly patients: a report of two clinical cases.
• Sterolins ABCG5 and ABCG8: regulators of whole body dietary sterols.
• Tannic acid synergizes the cytotoxicity of chemotherapeutic drugs in human cholangiocarcinoma by modulating drug efflux pathways.
• The MicroRNA let-7a modulates interleukin-6-dependent STAT-3 survival signaling in malignant human cholangiocytes.
• The liganding of glycolipid transfer protein is controlled by glycolipid acyl structure.
• The role of emotion in decision-making: a cognitive neuroeconomic approach towards understanding sexual risk behavior.
• Transient state kinetics of transcription elongation by T7 RNA polymerase.
• Trusted and relevant medical knowledge: the promise of information retrieval in biomedicine.
• When you come to a fork in the road, take it: strategy selection in order entry.
• Workflow modeling in critical care: piecing your own puzzle.
• p38 Mitogen-activated protein kinase mediates cell death and p21-activated kinase mediates cell survival during chemotherapeutic drug-induced mitotic arrest.