Conditional mutation of an essential putative glycoprotease eliminates autolysis in Staphylococcus aureus.

Conditional mutation of an essential putative glycoprotease eliminates autolysis in Staphylococcus aureus. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Conditional mutation of an essential putative glycoprotease eliminates autolysis in Staphylococcus aureus. Abstract Text:

Li Zheng,Chuanxin Yu,Kenneth Bayles, Lasa,Yinduo Ji,

Our previous studies demonstrated that a putative Staphylococcus aureus glycoprotease (Gcp) is essential for bacterial survival, indicating that Gcp may be a novel target for developing antibacterial agents. However, the biological function of Gcp is unclear. In order to elucidate the reason that Gcp is required for growth, we examined the role of Gcp in bacterial autolysis, which is an important biological process for bacterial growth. Using both a spacp-regulated gcp expression strain and a TetR-regulated gcp antisense expression strain, we found that the down-regulation of gcp expression can effectively inhibit Triton X-100-induced lysis, eliminate penicillin- and vancomycin-caused cell lysis, and dramatically increase tolerance to hydrolases. Moreover, we determined whether resistance to lysis is due to a defect in murein hydrolase activity by using a zymogram analysis. The results showed that the cell lysate of a down-regulated gcp expression mutant displayed several bands of decreased murein hydrolytic activity. Furthermore, we explored the potential mechanism of Gcp's involvement in autolysis and demonstrated that Gcp may function independently from several key autolysins (Atl, LytM, and LytN) and regulators (ArlRS, Mgr/Rat, and CidA). Taken together, the above results indicate that the essential Gcp is involved in the modification of substrates of murein hydrolases as well as in the regulation of expression and/or activity of some murein hydrolases, which, in turn, may play important roles in bacterial viability.

Conditional mutation of an essential putative glycoprotease eliminates autolysis in Staphylococcus aureus. Publishing Authors By Initials

L Zheng,C Yu,K Bayles,I Lasa,Y Ji,

For similar bacteria: gram-positive bacteria: gram-positive cocci: staphylococcaceae: staphylococcus: staphylococcus aureus research abstracts see: bacteria: gram-positive bacteria: gram-positive cocci: staphylococcaceae: staphylococcus: staphylococcus aureus research

PUBMED ID PMID:

MEDLINE DATE:

Conditional mutation of an essential putative glycoprotease eliminates autolysis in Staphylococcus aureus. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of bacteriology

VOLUME: 189

Page Numbers: 2734-42

Journal Abbreviation: J. Bacteriol.

ISSN: 0021-9193

DAY: 19

MONTH: 01

YEAR: 2007

Conditional mutation of an essential putative glycoprotease eliminates autolysis in Staphylococcus aureus. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985120

Conditional mutation of an essential putative glycoprotease eliminates autolysis in Staphylococcus aureus. Keywords Mesh Terms:

KEYWORDS: Staphylococcus aureus

MESH TERMS: growth & development

Chemical & Substance for Abstract: Conditional mutation of an essential putative glycoprotease eliminates autolysis in Staphylococcus aureus. Information

Substance Name: N-Acetylmuramoyl-L-alanine Amidase

Registry Number: EC 3.5.1.28

Grant and Affiliation Information for Conditional mutation of an essential putative glycoprotease eliminates autolysis in Staphylococcus aureus.

AFFILIATION: Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, 1971 Commonwealth Ave., St. Paul, MN 55108, USA.

Country: United States

AGENCY: United States NIAID

GRANT: AI065740

ACRONYM: AI

MEDLINETA: J Bacteriol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Conditional mutation of an essential putative glycoprotease eliminates autolysis in Staphylococcus aureus Related Publications

• Alpha-toxin interferes with integrin-mediated adhesion and internalization of Staphylococcus aureus by epithelial cells.
• Linkage and association studies of the susceptibility genes for type 2 diabetes.
• Peripheral signals in the control of feeding behavior.
• Unfolding new mechanisms of alcoholic liver disease in the endoplasmic reticulum.
• [Channelopathy of voltage-gated sodium channel]
• [Co-expression of human bone morphogenetic protein-2 and osteoprotegerin in myoblast C2C12]
• [Coordination bonding driving supramolecular self-assembly of tetraphenylporphyrinatozinc (II) with imidazolyl tailed porphyrinatoiron (III) studied by fluorescence spectra and 1H NMR]
• [Development and equivalence evaluation of monosyllable lists of mandarin speech test materials]
• [Development and equivalence evaluation of spondee lists of mandarin speech test materials]
• [Development of monoclonal antibody against fumonisin B1 and ELISA-kit of quantitative analysis for fumonisin B1]
• [Development of portable LED-based NIR integrity wheat component measuring apparatus]
• [Dual-wavelength method for analysis of overlapped high performance liquid chromatographic peaks with multiwavelength diode array detection]
• [Effects of hypothyroidism on apoptosis and the expression of Bcl-2 and Bax gene in the neonatal rat hippocampus neurons]
• [Effects of smoke inhalation injury on the phagocytic function of rat alveolar macrophage and on neutrophil apoptosis]
• [Expression of androgen receptor and estrogen receptor in carcinoma of larynx]
• [Expression of integrin-linked kinase is closely correlated with laryngeal squamous cell carcinomas]
• [Influences of bcr-abl gene vaccine on inoculated SP2/0/bcr-abl tumor cells in mice]
• [Nanomanipulation of sections of human tongue squamous cell carcinoma by atomic force microscopy]
• [Pattern of plasma sex steroid hormone levels during the breeding season of male and female skink: Eumeces chinensis]
• [Recombinant eukaryotic expression plasmid of bcr-abl gene fragment induces specific immune response in mice]
• [Remaining laryngeal flap reconstruction of the hypopharynx in pyriform sinus carcinoma resection]
• [Simultaneous determination of glucose, fructose and sucrose in aqueous solution by short-wavelength near infrared spectroscopy]
• [Spectroscopic investigation of the properties of axial coordination of tailed metalloporphyrins]
• [Spleen-preserving distal pancreatectomy in treatment of solid-pseudopapillary neoplasm]
• [Study of distribution of sinapine in commonly used crude drugs from cruciferous plants]
• [T staying not the single criterion of treating laryngeal cancer with surgical techniques]
• [The observation of palatopharyngeal histopathologic characteristic on the patients with obstructive sleep apnea-hypopnea syndrome]
• [The visible spectra study of the conformation equilibrium of iron-metal-free porphyrin dimers]
• [Transmission disequilibrium test for congenital dislocation of the hip and HOXB9 gene or COL1AI gene]
• [Two questions about "Understanding the hemorrhage due to late onset vitamin K deficiency"]