Concurrent splicing and transcription are not sufficient to enhance splicing efficiency.

Concurrent splicing and transcription are not sufficient to enhance splicing efficiency. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Concurrent splicing and transcription are not sufficient to enhance splicing efficiency. Abstract Text:

Denis Lazarev,James L Manley,

The concept of a tight integration of transcription and splicing of mRNA precursors has been supported with increasing evidence in recent years. However, the mechanism and functional consequences of this integration remain largely unknown. We have examined how these processes impact upon one another when they occur together in HeLa nuclear extract. While both processes do in fact occur in parallel reactions in the extracts, we found no evidence that one process affects the other, under a variety of conditions tested. For example, neither the kinetics nor efficiency of splicing is significantly enhanced by de novo RNA polymerase II-mediated transcription, relative to that of presynthesized RNA added exogenously to the extract. Our results indicate that the act of transcription by RNA polymerase II in vitro is not sufficient to enhance splicing of the newly made RNA.

Concurrent splicing and transcription are not sufficient to enhance splicing efficiency. Publishing Authors By Initials

D Lazarev,JL Manley,

For similar genetic processes: gene expression: transcription, genetic research abstracts see: genetic processes: gene expression: transcription, genetic research

PUBMED ID PMID:

MEDLINE DATE:

Concurrent splicing and transcription are not sufficient to enhance splicing efficiency. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: RNA (New York, N.Y.)

VOLUME: 13

Page Numbers: 1546-57

Journal Abbreviation: RNA

ISSN: 1355-8382

DAY: 13

MONTH: 07

YEAR: 2007

Concurrent splicing and transcription are not sufficient to enhance splicing efficiency. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9509184

Concurrent splicing and transcription are not sufficient to enhance splicing efficiency. Keywords Mesh Terms:

KEYWORDS: Transcription, Genetic

MESH TERMS: physiology

Chemical & Substance for Abstract: Concurrent splicing and transcription are not sufficient to enhance splicing efficiency. Information

Substance Name: RNA Polymerase II

Registry Number: EC 2.7.7.-

Grant and Affiliation Information for Concurrent splicing and transcription are not sufficient to enhance splicing efficiency.

AFFILIATION: Department of Biological Sciences, Columbia University, New York, NY 10027, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM 48259

ACRONYM: GM

MEDLINETA: RNA

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Concurrent splicing and transcription are not sufficient to enhance splicing efficiency Related Publications

• A complex signaling pathway regulates SRp38 phosphorylation and pre-mrna splicing in response to heat shock.
• A ported, proximally-cemented femoral stem for total hip arthroplasty. Development and clinical application.
• Aquaporins: role in cerebral edema and brain water balance.
• Atypical memory phenotype T cells with low homeostatic potential and impaired TCR signaling and regulatory T cell function in Foxn1Delta/Delta mutant mice.
• Concurrent splicing and transcription are not sufficient to enhance splicing efficiency.
• Diabetes mellitus in childhood; report of a local survey.
• Dose-response relationship of mannitol and intracranial pressure: a metaanalysis.
• Estimated average glucose derived from HbA(1c) (eAG): report from European Association for the Study of Diabetes (EASD), Amsterdam 2007.
• Gcm2 is required for the differentiation and survival of parathyroid precursor cells in the parathyroid/thymus primordia.
• Human capping enzyme promotes formation of transcriptional R loops in vitro.
• New approaches to physiological informatics in neurocritical care.
• New insights into mitotic chromosome condensation: a role for the prolyl isomerase pin1.
• Pin1 modulates RNA polymerase II activity during the transcription cycle.
• Prehospital hypoxia affects outcome in patients with traumatic brain injury: a prospective multicenter study.
• Protein-free spliceosomal snRNAs catalyze a reaction that resembles the first step of splicing.
• Relationship between brain tissue oxygen tension and CT perfusion: feasibility and initial results.
• Specific expression of lacZ and cre recombinase in fetal thymic epithelial cells by multiplex gene targeting at the Foxn1 locus.
• Sumoylation modulates the assembly and activity of the pre-mRNA 3' processing complex.
• Sumoylation regulates multiple aspects of mammalian poly(A) polymerase function.
• T cell development from kit-negative progenitors in the foxn1{delta}/{delta} mutant thymus.
• The RNA binding protein RNPS1 alleviates ASF/SF2 depletion-induced genomic instability.
• The impact of smoking-cessation intervention by multiple health professionals.
• The isolation and characterization of glycosaminoglycans in normal human serum.
• The multifunctional protein p54nrb/PSF recruits the exonuclease XRN2 to facilitate pre-mRNA 3' processing and transcription termination.
• The prolyl isomerase Pin1 functions in mitotic chromosome condensation.
• The role of lung function in brain tissue oxygenation following traumatic brain injury.
• The search for alternative splicing regulators: new approaches offer a path to a splicing code.
• Vancomycin use in patients requiring hemodialysis: a literature review.
• Variations in intracellular levels of TATA binding protein can affect specific genes by different mechanisms.
• hnRNP A1 functions with specificity in repression of SMN2 exon 7 splicing.