Concentration dependent selection of targets by an SR splicing regulator results in tissue-specific RNA processing.

Concentration dependent selection of targets by an SR splicing regulator results in tissue-specific RNA processing. Research Abstract Details 

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Concentration dependent selection of targets by an SR splicing regulator results in tissue-specific RNA processing. Abstract Text:

Junlin Qi,Shihuang Su,M Elaine McGuffin,William Mattox,

The splicing factor Transformer-2 (Tra2) is expressed almost ubiquitously in Drosophila adults, but participates in the tissue-specific regulation of splicing in several RNAs. In somatic tissues Tra2 participates in the activation of sex-specific splice sites in doublesex and fruitless pre-mRNAs. In the male germline it affects splicing of other transcripts and represses removal of the M1 intron from its own pre-mRNA. Here we test the hypothesis that the germline specificity of M1 repression is determined by tissue-specific differences in Tra2 concentration. We find that Tra2 is expressed at higher levels in primary spermatocytes of males than in other cell types. Increased Tra2 expression in other tissues reduces viability in a manner consistent with known dose-dependent effects of excessive Tra2 expression in the male germline. Somatic cells were found to be competent to repress M1 splicing if the level of Tra2 transcription was raised above endogenous concentrations. This suggests not only that M1 repression is restricted to the germline by a difference in Tra2 transcription levels but also that the protein's threshold concentration for M1 regulation differs from that of doublesex and fruitless RNAs. We propose that quantitative differences in regulator expression can give rise to cell-type-specific restrictions in splicing.

Concentration dependent selection of targets by an SR splicing regulator results in tissue-specific RNA processing. Publishing Authors By Initials

J Qi,S Su,ME McGuffin,W Mattox,

For similar genetic structures: genome: genome components: genes: transgenes research abstracts see: genetic structures: genome: genome components: genes: transgenes research

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Concentration dependent selection of targets by an SR splicing regulator results in tissue-specific RNA processing. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Nucleic acids research

VOLUME: 34

Page Numbers: 6256-63

Journal Abbreviation: Nucleic Acids Res.

ISSN: 1362-4962

DAY: 10

MONTH: 11

YEAR: 2006

Concentration dependent selection of targets by an SR splicing regulator results in tissue-specific RNA processing. Information

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LANGUAGE: eng

NlmUniqueID: 411011

Concentration dependent selection of targets by an SR splicing regulator results in tissue-specific RNA processing. Keywords Mesh Terms:

KEYWORDS: Transgenes

MESH TERMS: metabolism

Chemical & Substance for Abstract: Concentration dependent selection of targets by an SR splicing regulator results in tissue-specific RNA processing. Information

Substance Name: tra2 protein, Drosophila

Registry Number: 0

Grant and Affiliation Information for Concentration dependent selection of targets by an SR splicing regulator results in tissue-specific RNA processing.

AFFILIATION: Department of Molecular Genetics, The University of Texas M. D. Anderson Cancer Center and Genes and Development Graduate Program, The University of Texas Graduate School of Biomedical Sciences, Houston, TX 77030-4009, USA.

Country: England

AGENCY: United States NIGMS

GRANT: GM070892

ACRONYM: GM

MEDLINETA: Nucleic Acids Res

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