Concanavalin A-induced increase in the membrane fluidity of chicken erythrocytes.

Concanavalin A-induced increase in the membrane fluidity of chicken erythrocytes. Research Abstract Details 

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Concanavalin A-induced increase in the membrane fluidity of chicken erythrocytes. Abstract Text:

Y Ito,R Yoshimoto,T Irimura,M Setaka,H Shimizu,T Osawa,

To determine the fluidity of the membrane lipid phase, chicken erythrocytes were labeled with a stearic acid derivative spin label. When chicken erythrocytes were treated with concanavalin A (Con A), ESR spectra showed a change in the peaks of the labels in membrane lipids, indicating an increase of membrane fluidity. The degree of the increase in fluidity of the membrane lipid phase depended on the valency of the lectin used. Tetravalent Con A induced an increase of membrane fluidity at a concentration as low as 30 micrograms/ml, while a monovalent derivative of Con A did not affect membrane fluidity. This increase in membrane fluidity was observed within 10 min after the addition of Con A. If bound Con A was removed with methyl alpha-D-mannoside later than 60 min after its addition, a complete return of the fluidity to the normal level could not be observed. However, no change was found in the composition of phospholipids or in the fatty acid compositions of phosphatidylcholine and phosphatidylethanolamine of chicken erythrocytes after the addition of Con A, indicating that this increase in membrane fluidity is not caused by a change of lipid composition. The clustering of membrane receptors of chicken erythrocytes for Con A was demonstrated when the two-dimensional distribution of ferritin-conjugated Con A on the membranes was assayed by transmission electron microscopy. Furthermore, it was shown that major receptors for Con A of chicken erythrocytes were transmembrane glycoproteins having apparent molecular weights of 100K, 45, and 33K.

Concanavalin A-induced increase in the membrane fluidity of chicken erythrocytes. Publishing Authors By Initials

Y Ito,R Yoshimoto,T Irimura,M Setaka,H Shimizu,T Osawa,

For similar proteins: membrane proteins: receptors, cell surface: receptors, immunologic: receptors, mitogen: receptors, concanavalin a research abstracts see: proteins: membrane proteins: receptors, cell surface: receptors, immunologic: receptors, mitogen: receptors, concanavalin a research

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Concanavalin A-induced increase in the membrane fluidity of chicken erythrocytes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 86

Page Numbers: 1807-15

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Dec

YEAR: 1979

Concanavalin A-induced increase in the membrane fluidity of chicken erythrocytes. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Concanavalin A-induced increase in the membrane fluidity of chicken erythrocytes. Keywords Mesh Terms:

KEYWORDS: Receptors, Concanavalin A

MESH TERMS: metabolism

Chemical & Substance for Abstract: Concanavalin A-induced increase in the membrane fluidity of chicken erythrocytes. Information

Substance Name: Concanavalin A

Registry Number: 11028-71-0

Grant and Affiliation Information for Concanavalin A-induced increase in the membrane fluidity of chicken erythrocytes.

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Country: JAPAN

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MEDLINETA: J Biochem

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