Computational model of excitable cell indicates ATP free energy dynamics in response to calcium oscillations are undampened by cytosolic ATP buffers.

Computational model of excitable cell indicates ATP free energy dynamics in response to calcium oscillations are undampened by cytosolic ATP buffers. Research Abstract Details 

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Computational model of excitable cell indicates ATP free energy dynamics in response to calcium oscillations are undampened by cytosolic ATP buffers. Abstract Text:

R G P M van Stiphout,N A W van Riel,P J Verhoog,P A J Hilbers,K Nicolay,J A L Jeneson,

Mitochondria in excitable cells are recurrently exposed to pulsatile calcium gradients that activate cell function. Rapid calcium uptake by the mitochondria has previously been shown to cause uncoupling of oxidative phosphorylation. To test (i) if periodic nerve firing may cause oscillation of the cytosolic thermodynamic potential of ATP hydrolysis and (ii) if cytosolic adenylate (AK) and creatine kinase (CK) ATP buffering reactions dampen such oscillations, a lumped kinetic model of an excitable cell capturing major aspects of the physiology has been developed. Activation of ATP metabolism by low-frequency calcium pulses caused large oscillation of the cytosolic, but not mitochondrial ATP/ADP, ratio. This outcome was independent of net ATP synthesis or hydrolysis during mitochondrial calcium uptake. The AK/CK ATP buffering reactions dampened the amplitude and rate of cytosolic ATP/ADP changes on a timescale of seconds, but not milliseconds. These model predictions suggest that alternative sources of capacitance in neurons and striated muscles should be considered to protect ATP-free energy-driven cell functions.

Computational model of excitable cell indicates ATP free energy dynamics in response to calcium oscillations are undampened by cytosolic ATP buffers. Publishing Authors By Initials

RG van Stiphout,NA van Riel,PJ Verhoog,PA Hilbers,K Nicolay,JA Jeneson,

For similar nervous system: neurons research abstracts see: nervous system: neurons research

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Computational model of excitable cell indicates ATP free energy dynamics in response to calcium oscillations are undampened by cytosolic ATP buffers. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Systems biology

VOLUME: 153

Page Numbers: 405-8

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ISSN: 1741-2471

DAY: 7

MONTH: Sep

YEAR: 2006

Computational model of excitable cell indicates ATP free energy dynamics in response to calcium oscillations are undampened by cytosolic ATP buffers. Information

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LANGUAGE: eng

NlmUniqueID: 101232067

Computational model of excitable cell indicates ATP free energy dynamics in response to calcium oscillations are undampened by cytosolic ATP buffers. Keywords Mesh Terms:

KEYWORDS: Neurons

MESH TERMS: physiology

Chemical & Substance for Abstract: Computational model of excitable cell indicates ATP free energy dynamics in response to calcium oscillations are undampened by cytosolic ATP buffers. Information

Substance Name: Calcium

Registry Number: 7440-70-2

Grant and Affiliation Information for Computational model of excitable cell indicates ATP free energy dynamics in response to calcium oscillations are undampened by cytosolic ATP buffers.

AFFILIATION: Department of Biomedical Engineering, Eindhoven, University of Technology, Den Dolech 2, 5612 Eindhoven, The Netherlands.

Country: England

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MEDLINETA: Syst Biol (Stevenage)

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