Comprehensive genetic selection revealed essential bases in the peptidyl-transferase center.

Comprehensive genetic selection revealed essential bases in the peptidyl-transferase center. Research Abstract Details 

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Comprehensive genetic selection revealed essential bases in the peptidyl-transferase center. Abstract Text:

Neuza Satomi Sato,Naomi Hirabayashi,Ilana Agmon,Ada Yonath,Tsutomu Suzuki,

During protein synthesis, the ribosome catalyzes peptide-bond formation. Biochemical and structural studies revealed that conserved nucleotides in the peptidyl-transferase center (PTC) and its proximity may play a key role in peptide-bond formation; the exact mechanism involved remains unclear. To more precisely define the functional importance of the highly conserved residues, we used a systematic genetic method, which we named SSER (systematic selection of functional sequences by enforced replacement), that allowed us to identify essential nucleotides for ribosomal function from randomized rRNA libraries in Escherichia coli cells. These libraries were constructed by complete randomization of the critical regions in and around the PTC. The selected variants contained natural rRNA sequences from other organisms and organelles as well as unnatural functional sequences; hence providing insights into the functional roles played by these essential bases and suggesting how the universal catalytic mechanism of peptide-bond formation could evolve in all living organisms. Our results highlight essential bases and interactions, which are shaping the PTC architecture and guiding the motions of the tRNA terminus from the A to the P site, found to be crucial not only for the formation of the peptide bond but also for nascent chain elongation.

Comprehensive genetic selection revealed essential bases in the peptidyl-transferase center. Publishing Authors By Initials

NS Sato,N Hirabayashi,I Agmon,A Yonath,T Suzuki,

For similar investigative techniques: genetic techniques: sequence analysis: sequence analysis, dna research abstracts see: investigative techniques: genetic techniques: sequence analysis: sequence analysis, dna research

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Comprehensive genetic selection revealed essential bases in the peptidyl-transferase center. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 103

Page Numbers: 15386-91

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 10

MONTH: 10

YEAR: 2006

Comprehensive genetic selection revealed essential bases in the peptidyl-transferase center. Information

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LANGUAGE: eng

NlmUniqueID: 7505876

Comprehensive genetic selection revealed essential bases in the peptidyl-transferase center. Keywords Mesh Terms:

KEYWORDS: Sequence Analysis, DNA

MESH TERMS: methods

Chemical & Substance for Abstract: Comprehensive genetic selection revealed essential bases in the peptidyl-transferase center. Information

Substance Name: Peptidyl Transferases

Registry Number: EC 2.3.2.12

Grant and Affiliation Information for Comprehensive genetic selection revealed essential bases in the peptidyl-transferase center.

AFFILIATION: Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.

Country: United States

AGENCY: United States NIGMS

GRANT: GM34360

ACRONYM: GM

MEDLINETA: Proc Natl Acad Sci U S A

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